biostudies-literatureLei XNational Institute of Allergy and Infectious DiseasesNIAID NIH HHSSTARR Cancer ConsortiumResearch Foundation for the State University of New YorkMark Foundation For Cancer ResearchDOD Peer Reviewed Cancer Research ProgramG. Harold and Leila Y. Mathers FoundationNational Institute of General Medical SciencesNIGMS NIH HHSe011570https://www.ebi.ac.uk/biostudies/studies/S-EPMC12878199biostudies-literatureUnknown14(2)<h4>Background</h4>Colorectal cancer (CRC) is a leading cause of cancer-related death and remains a significant global health challenge. Cancer vaccines have emerged as a promising immunotherapy for long-term tumor control. While <i>Listeria monocytogenes</i> (<i>Lm</i>)-based intravenous vaccines can generate tumor-reactive CD8 T cells, clinical trial success has been limited. Here, we sought to determine whether <i>in vivo</i> targeting of gastrointestinal tissues with foodborne delivery of <i>Lm</i>-based cancer vaccines controlled tumor growth in murine models of CRC.<h4>Methods</h4>The ActA and InlB virulence genes were deleted from a mouse-adapted <i>Lm</i> strain expressing ovalbumin and containing an internalin A mutation (InlA^M <i>Lm</i>-ova) that allows epithelial cellJournal for immunotherapy of cancerOral immunization with &lt;i&gt;Listeria monocytogenes&lt;/i&gt; vaccine enhances immunotherapy for protective immunity in murine models of colorectal cancer.PMC12878199I13-0052N/AT32 GM14833120-028-EDVW81XWH-18-1-0217R01 AI172919R01AI172919Chu THBeyaz SOzler KAWestcott PMKChung CQiu ZLi XLei XZhang YSheridan BSYang RYu YBurgac MfalseOral immunization with &lt;i&gt;Listeria monocytogenes&lt;/i&gt; vaccine enhances immunotherapy for protective immunity in murine models of colorectal cancer.<h4>Background</h4>Colorectal cancer (CRC) is a leading cause of cancer-related death and remains a significant global health challenge. Cancer vaccines have emerged as a promising immunotherapy for long-term tumor control. While <i>Listeria monocytogenes</i> (<i>Lm</i>)-based intravenous vaccines can generate tumor-reactive CD8 T cells, clinical trial success has been limited. Here, we sought to determine whether <i>in vivo</i> targeting of gastrointestinal tissues with foodborne delivery of <i>Lm</i>-based cancer vaccines controlled tumor growth in murine models of CRC.<h4>Methods</h4>The ActA and InlB virulence genes were deleted from a mouse-adapted <i>Lm</i> strain expressing ovalbumin and containing an internalin A mutation (InlA^M <i>Lm</i>-ova) that allows epithelial cell2026-01-01T00:00:00Z2026 Feb2026-07-16T03:13:13.88Z2026-07-09T10:30:58.729ZS-EPMC128781994164426910.1136/jitc-2025-011570