{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Li JL"],"pubmed_abstract":["<h4>Introduction</h4>Xylanases are key catalysts for valorizing lignocellulosic biomass, yet many available enzymes lack sufficient thermal stability and exhibit suboptimal activity on complex substrates. To address these limitations, we combined enrichment culturing with metagenomic analysis to discover and characterize two novel GH10 family xylanases, Tc15-Xyn6 and Tc15-Xyn10, from the Qiaoquan geothermal area in Tengchong, Yunnan Province.<h4>Methods</h4>Following molecular cloning, heterologous expression, and purification by Ni<sup>2+</sup>-chelating affinity chromatography, both enzymes were comprehensively profiled.<h4>Results</h4>Tc15-Xyn6 displayed optimal activity at 65 °C and pH 6.6 with a half-life of 2 h at 65 °C, while Tc15-Xyn10 exhibited optimal activity at 60 °C and pH 6.0"],"journal":["Frontiers in microbiology"],"pagination":["1731615"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12880817"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of thermophilic xylanases from Tengchong Qiaoquan hot spring for lignocellulose bioprocessing and prebiotic production."],"pmcid":["PMC12880817"],"pubmed_authors":["Chen XQ","Li LH","Xie KQ","Yin YR","Yang ZF","Hu W","Zhang YW","Zheng YY","Li JL","Yang LQ","Phurbu D","Sun J"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of thermophilic xylanases from Tengchong Qiaoquan hot spring for lignocellulose bioprocessing and prebiotic production.","description":"<h4>Introduction</h4>Xylanases are key catalysts for valorizing lignocellulosic biomass, yet many available enzymes lack sufficient thermal stability and exhibit suboptimal activity on complex substrates. To address these limitations, we combined enrichment culturing with metagenomic analysis to discover and characterize two novel GH10 family xylanases, Tc15-Xyn6 and Tc15-Xyn10, from the Qiaoquan geothermal area in Tengchong, Yunnan Province.<h4>Methods</h4>Following molecular cloning, heterologous expression, and purification by Ni<sup>2+</sup>-chelating affinity chromatography, both enzymes were comprehensively profiled.<h4>Results</h4>Tc15-Xyn6 displayed optimal activity at 65 °C and pH 6.6 with a half-life of 2 h at 65 °C, while Tc15-Xyn10 exhibited optimal activity at 60 °C and pH 6.0","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-07-15T06:37:09.137Z","creation":"2026-07-02T03:08:30.879Z"},"accession":"S-EPMC12880817","cross_references":{"pubmed":["41657984"],"doi":["10.3389/fmicb.2025.1731615"]}}