{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lewis J"],"funding":["NHLBI NIH HHS"],"pagination":["11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12893397"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(3)"],"pubmed_abstract":["<h4>Background</h4>Human induced pluripotent stem cell cardiomyocytes (hiPSC-CMs) allow for high-throughput evaluation of cardiomyocyte (CM) physiology in health and disease. While multimodality testing provides a large breadth of information related to electrophysiology, contractility, and intracellular signaling in small populations of iPSC-CMs, current technologies for analyzing these parameters are expensive and resource-intensive.<h4>Methods</h4>We have designed a novel 2D/3D hybrid organoid system that can harness optical imaging techniques to assess electromechanical properties and calcium dynamics across CMs in a high-throughput manner. We validated our methods using a doxorubicin-based system, as the drug has well-characterized cardiotoxic, pro-arrhythmic effects.<h4>Results</h4>T"],"journal":["Therapeutics"],"pubmed_title":["Novel 2D/3D Hybrid Organoid System for High-Throughput Drug Screening in iPSC Cardiomyocytes."],"pmcid":["PMC12893397"],"funding_grant_id":["K08 HL161440"],"pubmed_authors":["Lewis J","Yaseen B","Saraf A","Wu H"],"additional_accession":[]},"is_claimable":false,"name":"Novel 2D/3D Hybrid Organoid System for High-Throughput Drug Screening in iPSC Cardiomyocytes.","description":"<h4>Background</h4>Human induced pluripotent stem cell cardiomyocytes (hiPSC-CMs) allow for high-throughput evaluation of cardiomyocyte (CM) physiology in health and disease. While multimodality testing provides a large breadth of information related to electrophysiology, contractility, and intracellular signaling in small populations of iPSC-CMs, current technologies for analyzing these parameters are expensive and resource-intensive.<h4>Methods</h4>We have designed a novel 2D/3D hybrid organoid system that can harness optical imaging techniques to assess electromechanical properties and calcium dynamics across CMs in a high-throughput manner. We validated our methods using a doxorubicin-based system, as the drug has well-characterized cardiotoxic, pro-arrhythmic effects.<h4>Results</h4>T","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-07-08T03:09:18.269Z","creation":"2026-07-08T03:08:20.258Z"},"accession":"S-EPMC12893397","cross_references":{"pubmed":["41684727"],"doi":["10.3390/therapeutics2030011"]}}