biostudies-literatureUnknown17Guo YWhile probiotics like <i>Enterococcus faecium</i> are known for gut health benefits, their potential anti-aging effects are poorly understood. This study investigated whether <i>E. faecium</i> fermentation broth delays aging and explored its mechanisms. Using a mouse model, lifespan assays suggested that the <i>E. faecium fermentation</i> broth may contribute to lifespan extension, indicating anti-aging properties. Microbiome analysis showed it modulated gut microbiota, increasing beneficial <i>Akkermansia</i> abundance. Key active components identified included myo-inositol (promoting hair follicle growth), D-ribose, and secreted proteins. While myo-inositol increased the abundance of <i>Lactobacillus reuteri</i> and <i>Lactobacillus johnsonii</i>, it did not increase <i>Akkermansia</i>. Frontiers in microbiology1680593https://www.ebi.ac.uk/biostudies/studies/S-EPMC12894319biostudies-literature&lt;i&gt;Enterococcus faecium&lt;/i&gt; secreted the NlpC/P60 family protein to enhance host immunity and indirectly increases &lt;i&gt;Akkermansia muciniphila&lt;/i&gt; for slowing aging.PMC12894319Yang XZhang FGuo YFu WXu CZou SLi Rfalse&lt;i&gt;Enterococcus faecium&lt;/i&gt; secreted the NlpC/P60 family protein to enhance host immunity and indirectly increases &lt;i&gt;Akkermansia muciniphila&lt;/i&gt; for slowing aging.While probiotics like <i>Enterococcus faecium</i> are known for gut health benefits, their potential anti-aging effects are poorly understood. This study investigated whether <i>E. faecium</i> fermentation broth delays aging and explored its mechanisms. Using a mouse model, lifespan assays suggested that the <i>E. faecium fermentation</i> broth may contribute to lifespan extension, indicating anti-aging properties. Microbiome analysis showed it modulated gut microbiota, increasing beneficial <i>Akkermansia</i> abundance. Key active components identified included myo-inositol (promoting hair follicle growth), D-ribose, and secreted proteins. While myo-inositol increased the abundance of <i>Lactobacillus reuteri</i> and <i>Lactobacillus johnsonii</i>, it did not increase <i>Akkermansia</i>. 2026-01-01T00:00:00Z20262026-07-15T16:39:37.517Z2026-07-07T03:08:34.31ZS-EPMC128943194169594210.3389/fmicb.2026.1680593