biostudies-literatureXu JSwiss National Science Foundation1344https://www.ebi.ac.uk/biostudies/studies/S-EPMC12897804biostudies-literatureUnknown27(3)The transcription factor DMP1 is a positive regulator of the tumor suppressor ARF, which in turn controls cell-cycle progression and/or apoptosis through p53. Given the role of DMP1 in the ARF-p53 pathway, we investigated whether the p53 transcription factor might regulate DMP1 expression. We found that endogenous human (h)DMP1 mRNA levels were significantly decreased upon induction of the temperature-sensitive p53Val135 in normal fibroblasts. Consistent with this observation, a p53 knockdown in MCF7 breast cancer cells resulted in increased hDMP1 mRNA and protein levels. At the molecular level, we found that p53 directly repressed the hDMP1 promoter activity by up to 90%. This repression was not mediated by p53 binding to the two putative p53-binding sites International journal of molecular sciencesIdentification of DMP1 as Novel p53 Repressed Transcriptional Target.PMC1289780417321931003031003A_173219197786310030_197786Zlobec ITorbett BEArvidsson GTschan MPBritschgi CKrauer DXu JfalseIdentification of DMP1 as Novel p53 Repressed Transcriptional Target.The transcription factor DMP1 is a positive regulator of the tumor suppressor ARF, which in turn controls cell-cycle progression and/or apoptosis through p53. Given the role of DMP1 in the ARF-p53 pathway, we investigated whether the p53 transcription factor might regulate DMP1 expression. We found that endogenous human (h)DMP1 mRNA levels were significantly decreased upon induction of the temperature-sensitive p53Val135 in normal fibroblasts. Consistent with this observation, a p53 knockdown in MCF7 breast cancer cells resulted in increased hDMP1 mRNA and protein levels. At the molecular level, we found that p53 directly repressed the hDMP1 promoter activity by up to 90%. This repression was not mediated by p53 binding to the two putative p53-binding sites 2026-01-01T00:00:00Z2026 Jan2026-07-08T03:12:39.446Z2026-07-08T03:08:30.384ZS-EPMC128978044168376610.3390/ijms27031344