biostudies-literatureWang CThe Open Foundation of Key Laboratory of Taihu Basin Water Resources Management, Ministry of Water ResourcesNational Natural Science Foundation of China499https://www.ebi.ac.uk/biostudies/studies/S-EPMC12899096biostudies-literatureUnknown15(3)Thermophilic cyanobacteria are key models for thermotolerance and a promising source of thermophilic bioresources. Yet the subcellular basis of their stress resilience remains poorly resolved. Here, we focus on intracellular polyphosphate (polyP)-rich granules, termed "stabilisomes," which have been implicated in stress adaptation. The lack of a high-purity, structure-preserving isolation method has been a major technical bottleneck hindering the elucidation of this resilience mechanism. This study describes a robust, structure-preserving purification strategy, boosting the granule-to-protein yield by over 10,000-fold compared with conventional methods. The specificity and structural integrity of this method are supported by the specific enrichment of complex proteomic (937 proteins) and mPlants (Basel, Switzerland)High-Purity Isolation of Polyphosphate-Rich Stabilisomes Defines Their Conserved Chemical Architecture in Thermophilic Cyanobacteria.PMC12899096Yk922001-C24230717241871082Wang MYang LWang CZhou CSong XYin JfalseHigh-Purity Isolation of Polyphosphate-Rich Stabilisomes Defines Their Conserved Chemical Architecture in Thermophilic Cyanobacteria.Thermophilic cyanobacteria are key models for thermotolerance and a promising source of thermophilic bioresources. Yet the subcellular basis of their stress resilience remains poorly resolved. Here, we focus on intracellular polyphosphate (polyP)-rich granules, termed "stabilisomes," which have been implicated in stress adaptation. The lack of a high-purity, structure-preserving isolation method has been a major technical bottleneck hindering the elucidation of this resilience mechanism. This study describes a robust, structure-preserving purification strategy, boosting the granule-to-protein yield by over 10,000-fold compared with conventional methods. The specificity and structural integrity of this method are supported by the specific enrichment of complex proteomic (937 proteins) and m2026-01-01T00:00:00Z2026 Feb2026-07-06T03:22:26.84Z2026-07-06T03:11:12.815ZS-EPMC128990964168166210.3390/plants15030499