biostudies-literatureUnknown16(1)Chen X<h4>Background</h4>As the most important pathophysiological process of obstructive sleep apnea (OSA), chronic intermittent hypoxia (CIH) could cause cognitive impairment. Our previous study showed that Shengmai Dihuang Decoction (SDD) improved CIH-induced cognitive impairment. However, the precise underlying mechanism remains unclear.<h4>Objective</h4>This study aims to investigate the role of the insulin-like growth factor 1 (IGF1)/insulin-like growth factor 1 receptor (IGFR) signaling pathway in improving CIH-induced neuronal injury by SDD treatment.<h4>Methods</h4>In this study, HT22 cells were cultured under CIH conditions (O₂: 21-0.1 %, 6 cycles/h). Cell ability was assessed by CCK-8 assay, and Mitochondrial damage was detected by transmission electron microscopy (TEM), mitJournal of traditional and complementary medicine31-45https://www.ebi.ac.uk/biostudies/studies/S-EPMC12902252biostudies-literatureRole of IGF1/IGFR signaling pathway in neuroprotective effect of Shengmai Dihuang Decoction on CIH-induced cognitive impairment.PMC12902252Sun MFan XQi KLi DSi JJi EChen XYang SfalseRole of IGF1/IGFR signaling pathway in neuroprotective effect of Shengmai Dihuang Decoction on CIH-induced cognitive impairment.<h4>Background</h4>As the most important pathophysiological process of obstructive sleep apnea (OSA), chronic intermittent hypoxia (CIH) could cause cognitive impairment. Our previous study showed that Shengmai Dihuang Decoction (SDD) improved CIH-induced cognitive impairment. However, the precise underlying mechanism remains unclear.<h4>Objective</h4>This study aims to investigate the role of the insulin-like growth factor 1 (IGF1)/insulin-like growth factor 1 receptor (IGFR) signaling pathway in improving CIH-induced neuronal injury by SDD treatment.<h4>Methods</h4>In this study, HT22 cells were cultured under CIH conditions (O₂: 21-0.1 %, 6 cycles/h). Cell ability was assessed by CCK-8 assay, and Mitochondrial damage was detected by transmission electron microscopy (TEM), mit2026-01-01T00:00:00Z2026 Jan2026-07-15T21:59:56.31Z2026-07-09T10:20:11.916ZS-EPMC129022524169673910.1016/j.jtcme.2024.11.014