biostudies-literatureCai YBasic Research Program of JiangsuExcellence Research Group Program of NSFCNational Key R&D Program of ChinaResearch Funds of Hangzhou Institute for Advanced Study, UCASZhejiang Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaMajor Innovation Projects in Shandong ProvinceNational Key Research and Development Program of ChinaTaishan Scholars Program for Young Experts of Shandong Provinceof Shandong Provincee17423https://www.ebi.ac.uk/biostudies/studies/S-EPMC12903998biostudies-literatureUnknown13(9)Eukaryotic translation initiation factor 5A (eIF5A) facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood. Here eIF5A highly expressed in diverse spermatogenic cell types are found. Conditional knockout of Eif5a (SKO) causes complete infertility in male mice due to round spermatid arrest. Interestingly, eIF5A deletion severely compromises chromocenter integrity in round spermatids. Proteomic profiling reveals widespread dysregulation in eIF5A-deficient round spermatids, downregulated proteins are enriched for chromatin-associated functions, likely contributing to chromocenter dysfunction. Notably, ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) analysis shows increased chAdvanced science (Weinheim, Baden-Wurttemberg, Germany)EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis.PMC12903998LZ23C050001820716992025HLAS-ZL00832271347325882012023HIAS-Y022824716442024YFA1108100BK20240427823716192021ZDSYS16tsqn20210319282501941Liu HLi HBao ZQi HKong FZhang MLiu WGuan YLi TJiang XFang QCai YChen ZJWang WChen XWang XfalseEIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis.Eukaryotic translation initiation factor 5A (eIF5A) facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood. Here eIF5A highly expressed in diverse spermatogenic cell types are found. Conditional knockout of Eif5a (SKO) causes complete infertility in male mice due to round spermatid arrest. Interestingly, eIF5A deletion severely compromises chromocenter integrity in round spermatids. Proteomic profiling reveals widespread dysregulation in eIF5A-deficient round spermatids, downregulated proteins are enriched for chromatin-associated functions, likely contributing to chromocenter dysfunction. Notably, ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) analysis shows increased ch2026-01-01T00:00:00Z2026 Feb2026-07-15T15:38:10.944Z2026-07-06T03:11:39.966ZS-EPMC129039984148681610.1002/advs.202517423