{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guzel MA"],"funding":["Inönü Üniversitesi"],"pagination":["6276"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12905129"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Hypericum perforatum L. (St. John's wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cy"],"journal":["Scientific reports"],"pubmed_title":["Integrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays."],"pmcid":["PMC12905129"],"funding_grant_id":["4221"],"pubmed_authors":["Menevse IN","Kolac T","Ugur Y","Dundar M","Zengin R","Guzel A","Guzel MA"],"additional_accession":[]},"is_claimable":false,"name":"Integrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays.","description":"Hypericum perforatum L. (St. John's wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cy","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-15T21:31:16.068Z","creation":"2026-07-09T10:14:13.074Z"},"accession":"S-EPMC12905129","cross_references":{"pubmed":["41593177"],"doi":["10.1038/s41598-026-36793-8"]}}