biostudies-literatureGuzel MAInönü Üniversitesi6276https://www.ebi.ac.uk/biostudies/studies/S-EPMC12905129biostudies-literatureUnknown16(1)Hypericum perforatum L. (St. John's wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cyScientific reportsIntegrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays.PMC129051294221Menevse INKolac TUgur YDundar MZengin RGuzel AGuzel MAfalseIntegrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays.Hypericum perforatum L. (St. John's wort) is a medicinal plant known for its diverse secondary metabolites and pharmacological potential. This study provides a comprehensive evaluation of the phytochemical composition and bioactivities of methanolic extracts from wild H. perforatum. LC-MS/MS analysis identified 36 compounds, including phenolic acids (chlorogenic acid, protocatechuic acid) and flavonoids (rutin, isoquercitrin, quercetin), with the first-time detection of genkwanin, vicenin-2, schaftoside, and afzelin, to the best of our knowledge. The extract demonstrated significant antioxidant activity, with a total phenolic content of 203.04 mg GAE/g DW and strong radical scavenging activity in DPPH (544.78 mg TE/g DW) and ABTS (312.15 mg TE/g DW) assays. In vitro assays showed marked cy2026-01-01T00:00:00Z2026 Jan2026-07-15T21:31:16.068Z2026-07-09T10:14:13.074ZS-EPMC129051294159317710.1038/s41598-026-36793-8