{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peng F"],"funding":["NHLBI NIH HHS","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)"],"pagination":["1636"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12905394"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["Single-cell RNA sequencing (scRNA-seq) has identified intermediate epithelial states in pulmonary fibrosis, including KRT5-/KRT17+ aberrant basaloid cells in humans and Krt8+ alveolar differentiation intermediates (ADIs) in mice. Their functional contributions to fibrogenesis, however, remain unclear. Here, we introduce an RNA-sensing-dependent protein translation technology that enables selective targeting of Krt8+ ADI cells in vitro and in vivo. Transcriptomic analysis revealed Small Proline-Rich Protein 1 A (SPRR1A) mRNA as a shared marker of murine Krt8+ ADIs and human KRT5-/KRT17+ basaloid cells, distinguishing them from other lung cell populations. Using programmable RNA sensors, we demonstrated selective EGFP-labeling of Krt8+ ADI cells in vivo, which faithfully recapitulated their "],"journal":["Nature communications"],"pubmed_title":["Transcriptomic signature-guided depletion of intermediate alveolar epithelial cells ameliorates pulmonary fibrosis in mice."],"pmcid":["PMC12905394"],"funding_grant_id":["R01 HL156973","R01 HL139584","HL174994","R01 HL174994","HL156973","HL139584"],"pubmed_authors":["Saito S","Zheng Z","He C","Rosas IO","Aliyari S","Lasky JA","Zhou Y","Shan D","Thannickal VJ","Peng F","Sabharwal A","Yin Q","Jiang CS"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic signature-guided depletion of intermediate alveolar epithelial cells ameliorates pulmonary fibrosis in mice.","description":"Single-cell RNA sequencing (scRNA-seq) has identified intermediate epithelial states in pulmonary fibrosis, including KRT5-/KRT17+ aberrant basaloid cells in humans and Krt8+ alveolar differentiation intermediates (ADIs) in mice. Their functional contributions to fibrogenesis, however, remain unclear. Here, we introduce an RNA-sensing-dependent protein translation technology that enables selective targeting of Krt8+ ADI cells in vitro and in vivo. Transcriptomic analysis revealed Small Proline-Rich Protein 1 A (SPRR1A) mRNA as a shared marker of murine Krt8+ ADIs and human KRT5-/KRT17+ basaloid cells, distinguishing them from other lung cell populations. Using programmable RNA sensors, we demonstrated selective EGFP-labeling of Krt8+ ADI cells in vivo, which faithfully recapitulated their ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-15T22:10:17.526Z","creation":"2026-07-09T10:19:59.896Z"},"accession":"S-EPMC12905394","cross_references":{"pubmed":["41519994"],"doi":["10.1038/s41467-026-68354-y"]}}