{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Deshpande M"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","NIH NIGMS","NIGMS NIH HHS","Interface"],"pagination":["100292"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12906330"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38"],"pubmed_abstract":["The inhibition of a specific protein-protein interaction is often difficult to achieve in targeted drug design. We report the development and optimization of a general-purpose, readily implemented enzyme-linked immunosorbent assay (ELISA) for high-throughput screening to identify small-molecule inhibitors of protein interactions. This ELISA does not involve the use of any capture antibodies, probes, or compounds coated on the plate and represents a general strategy to identify inhibitors of a given protein-protein interaction. We demonstrate its utility in blocking lysinoalanine crosslinking between subunits of the spirochete flagellar hook by targeting the native form of the FlgE protein, which differs from the strategies used in previous assays. The flagellar hook protein FlgE self-catal"],"journal":["SLAS discovery : advancing life sciences R & D"],"pubmed_title":["An ELISA for discovering protein-protein interaction inhibitors: Blocking lysinoalanine crosslinking between subunits of the spirochete flagellar hook as a test case."],"pmcid":["PMC12906330"],"funding_grant_id":["T32 GM138826","R01 AI078958","R01 AI148844","R01AI148844","R01AI078958","T32GM138826"],"pubmed_authors":["Crane BR","Lynch MJ","Li C","Kurniyati K","Deshpande M"],"additional_accession":[]},"is_claimable":false,"name":"An ELISA for discovering protein-protein interaction inhibitors: Blocking lysinoalanine crosslinking between subunits of the spirochete flagellar hook as a test case.","description":"The inhibition of a specific protein-protein interaction is often difficult to achieve in targeted drug design. We report the development and optimization of a general-purpose, readily implemented enzyme-linked immunosorbent assay (ELISA) for high-throughput screening to identify small-molecule inhibitors of protein interactions. This ELISA does not involve the use of any capture antibodies, probes, or compounds coated on the plate and represents a general strategy to identify inhibitors of a given protein-protein interaction. We demonstrate its utility in blocking lysinoalanine crosslinking between subunits of the spirochete flagellar hook by targeting the native form of the FlgE protein, which differs from the strategies used in previous assays. The flagellar hook protein FlgE self-catal","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-15T22:18:30.793Z","creation":"2026-07-09T10:20:25.688Z"},"accession":"S-EPMC12906330","cross_references":{"pubmed":["41308746"],"doi":["10.1016/j.slasd.2025.100292"]}}