{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Wang Y"],"pubmed_abstract":["Calf diarrhea is a common gastrointestinal disease that usually occurs within one month of birth. The disease causes the greatest economic losses to the cattle industry. Currently, a variety of diagnostic methods have been developed for calf diarrhea infections. However, existing methods are still unsatisfactory in terms of sensitivity, specificity, simplicity, cost, and speed.To provide a more sensitive, specific, simpler, and faster detection method, we recently developed an RPA-CRISPR/Cas12a assay that can detect BVDV, BCoV, BRV, and ETEC infections in cattle on-site. Testing for each pathogen is performed in a single test tube, without the need to open the tube in the middle, and can be completed in under 50 minutes.The RPA-CRISPR/Cas12a assay can detect BVDV, BCoV, BRV, and ETEC at co"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["1734185"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12907351"],"repository":["biostudies-literature"],"pubmed_title":["Sensitive, specific, and rapid on-site detection of calf diarrhea pathogens using the RPA-CRISPR/Cas 12a assay."],"pmcid":["PMC12907351"],"pubmed_authors":["Zhang F","Diao Y","Zhang T","Wang W","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"Sensitive, specific, and rapid on-site detection of calf diarrhea pathogens using the RPA-CRISPR/Cas 12a assay.","description":"Calf diarrhea is a common gastrointestinal disease that usually occurs within one month of birth. The disease causes the greatest economic losses to the cattle industry. Currently, a variety of diagnostic methods have been developed for calf diarrhea infections. However, existing methods are still unsatisfactory in terms of sensitivity, specificity, simplicity, cost, and speed.To provide a more sensitive, specific, simpler, and faster detection method, we recently developed an RPA-CRISPR/Cas12a assay that can detect BVDV, BCoV, BRV, and ETEC infections in cattle on-site. Testing for each pathogen is performed in a single test tube, without the need to open the tube in the middle, and can be completed in under 50 minutes.The RPA-CRISPR/Cas12a assay can detect BVDV, BCoV, BRV, and ETEC at co","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026","modification":"2026-07-16T05:10:29.831Z","creation":"2026-07-09T10:36:37.92Z"},"accession":"S-EPMC12907351","cross_references":{"pubmed":["41704956"],"doi":["10.3389/fcimb.2026.1734185"]}}