{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gopinath D"],"funding":["Ajman University"],"pagination":["101410"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12907499"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(2)"],"pubmed_abstract":["<h4>Objective</h4>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <i>Fusobacterium nucleatum</i> (Fn) and <i>Porphyromonas gingivalis</i> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).<h4>Materials and methods</h4>HCT 116 cells were infected with <i>Fn</i> or <i>Pg</i> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate "],"journal":["Journal of oral biology and craniofacial research"],"pubmed_title":["Differential effect of Periodontal pathogen &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt; and &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; on modulation of specific partial-EMT genes in colorectal cancer cells."],"pmcid":["PMC12907499"],"funding_grant_id":["IRC- 2024-25-S-046","2023-IRG-DEN-11"],"pubmed_authors":["Gopinath D","Li Z","Selvakumar B","Sekar P","Mansoor Mohammed M"],"additional_accession":[]},"is_claimable":false,"name":"Differential effect of Periodontal pathogen &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt; and &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; on modulation of specific partial-EMT genes in colorectal cancer cells.","description":"<h4>Objective</h4>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <i>Fusobacterium nucleatum</i> (Fn) and <i>Porphyromonas gingivalis</i> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).<h4>Materials and methods</h4>HCT 116 cells were infected with <i>Fn</i> or <i>Pg</i> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Mar-Apr","modification":"2026-07-16T05:07:29.391Z","creation":"2026-07-10T03:08:46.51Z"},"accession":"S-EPMC12907499","cross_references":{"pubmed":["41704400"],"doi":["10.1016/j.jobcr.2026.101410"]}}