biostudies-literatureGopinath DAjman University101410https://www.ebi.ac.uk/biostudies/studies/S-EPMC12907499biostudies-literatureUnknown16(2)<h4>Objective</h4>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <i>Fusobacterium nucleatum</i> (Fn) and <i>Porphyromonas gingivalis</i> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).<h4>Materials and methods</h4>HCT 116 cells were infected with <i>Fn</i> or <i>Pg</i> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate Journal of oral biology and craniofacial researchDifferential effect of Periodontal pathogen &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt; and &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; on modulation of specific partial-EMT genes in colorectal cancer cells.PMC12907499IRC- 2024-25-S-0462023-IRG-DEN-11Gopinath DLi ZSelvakumar BSekar PMansoor Mohammed MfalseDifferential effect of Periodontal pathogen &lt;i&gt;Fusobacterium nucleatum&lt;/i&gt; and &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; on modulation of specific partial-EMT genes in colorectal cancer cells.<h4>Objective</h4>While the epithelial-mesenchymal transition (EMT) is known to promote cancer stemness and metastasis, a hybrid partial EMT (p-EMT) state has recently been identified. This study examined the influence of <i>Fusobacterium nucleatum</i> (Fn) and <i>Porphyromonas gingivalis</i> (Pg) on p-EMT gene expression and their prognostic relevance in colorectal cancer (CRC).<h4>Materials and methods</h4>HCT 116 cells were infected with <i>Fn</i> or <i>Pg</i> at an MOI of 100. Expression of p-EMT markers (SPP1, EMP3, INHBA, THBS2, ITGA5, P4HA2) and EMT-markers (ZEB1, SNAI1) was analyzed via RT-PCR after 6 hours (h) and 24 h. Cell migration was assessed using a scratch assay. TCGA data were used to validate gene expression in CRC patients, and Kaplan-Meier analysis was used to evaluate 2026-01-01T00:00:00Z2026 Mar-Apr2026-07-16T05:07:29.391Z2026-07-10T03:08:46.51ZS-EPMC129074994170440010.1016/j.jobcr.2026.101410