biostudies-literatureUnknown7Harinisri KIndustrial effluent treatment relies on chemical coagulants, which incur higher costs and generate toxic sludge. This study isolated and identified bioflocculant-producing <i>Bacillus mycoides</i> (S39) strain and enhanced yield using one-factor-at-a-time optimization. Characterization studies of purified bioflocculant reveal a crystalline polysaccharide bearing amine, hydroxyl, and carboxylate groups that drive adsorption and polymer bridging. The bioflocculant exhibited flocculation activities of 95.56 % for textile effluent and 92.84 % for steel wastewater with Ca²⁺ activation. Bioflocculant remained stable across pH and temperature. Kinetics followed a pseudo-first-order model, yielding rate constants, an optimized dosage, and assistance with sizing. Hemolysis (<5 %) and viaBiotechnology notes (Amsterdam, Netherlands)1-15https://www.ebi.ac.uk/biostudies/studies/S-EPMC12907645biostudies-literatureCharacterization and production of a &lt;i&gt;Bacillus mycoides&lt;/i&gt; Bioflocculant for sustainable effluent treatment.PMC12907645Harinisri KThamarai Selvi BfalseCharacterization and production of a &lt;i&gt;Bacillus mycoides&lt;/i&gt; Bioflocculant for sustainable effluent treatment.Industrial effluent treatment relies on chemical coagulants, which incur higher costs and generate toxic sludge. This study isolated and identified bioflocculant-producing <i>Bacillus mycoides</i> (S39) strain and enhanced yield using one-factor-at-a-time optimization. Characterization studies of purified bioflocculant reveal a crystalline polysaccharide bearing amine, hydroxyl, and carboxylate groups that drive adsorption and polymer bridging. The bioflocculant exhibited flocculation activities of 95.56 % for textile effluent and 92.84 % for steel wastewater with Ca²⁺ activation. Bioflocculant remained stable across pH and temperature. Kinetics followed a pseudo-first-order model, yielding rate constants, an optimized dosage, and assistance with sizing. Hemolysis (<5 %) and via2026-01-01T00:00:00Z20262026-07-16T05:01:21.324Z2026-07-09T13:10:25.341ZS-EPMC129076454170452710.1016/j.biotno.2026.01.001