{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Sun W"],"pubmed_abstract":["l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain <i>Klebsiella oxytoca</i> LKO-14 was constructed based on an l-valine producer <i>K</i>. <i>oxytoca</i> VKO-9. The exogenous l-leucine biosynthesis pathway was introduced to achieve l-leucine accumulation and decrease l-valine production. Modifying l-leucine transport system and optimizing copy numbers of genes including <i>CgleuA</i> <sup><i>M</i></sup> encoding l-leucine insensitive isopropylmalate synthase, <i>EcleuCD</i> encoding isopropylmalate isomerase, <i>EcleuB</i> encoding isopropylmalate dehydrogenase, <i>NopheDH</i> encoding phenylalanine dehydrogenase, were conducted to increase l-leucine production. The expression of <i>budB</i> encoding α-acetolactate synth"],"journal":["Synthetic and systems biotechnology"],"pagination":["163-171"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12907686"],"repository":["biostudies-literature"],"pubmed_title":["Multistep metabolic engineered &lt;i&gt;Klebsiella oxytoca&lt;/i&gt; for efficient l-leucine production."],"pmcid":["PMC12907686"],"pubmed_authors":["Xu P","Liu M","Kang Q","Lu C","Sun W","Kang Z","Gao C","Zhou Z","Meng W","Liu Y","Ma C","Tan X","Wang S","Gong L","Yang Q"],"additional_accession":[]},"is_claimable":false,"name":"Multistep metabolic engineered &lt;i&gt;Klebsiella oxytoca&lt;/i&gt; for efficient l-leucine production.","description":"l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain <i>Klebsiella oxytoca</i> LKO-14 was constructed based on an l-valine producer <i>K</i>. <i>oxytoca</i> VKO-9. The exogenous l-leucine biosynthesis pathway was introduced to achieve l-leucine accumulation and decrease l-valine production. Modifying l-leucine transport system and optimizing copy numbers of genes including <i>CgleuA</i> <sup><i>M</i></sup> encoding l-leucine insensitive isopropylmalate synthase, <i>EcleuCD</i> encoding isopropylmalate isomerase, <i>EcleuB</i> encoding isopropylmalate dehydrogenase, <i>NopheDH</i> encoding phenylalanine dehydrogenase, were conducted to increase l-leucine production. The expression of <i>budB</i> encoding α-acetolactate synth","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Sep","modification":"2026-07-16T05:17:38.641Z","creation":"2026-07-09T13:10:20.775Z"},"accession":"S-EPMC12907686","cross_references":{"pubmed":["41704463"],"doi":["10.1016/j.synbio.2026.01.024"]}}