{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xin C"],"funding":["the "Rising-Star Program" of “Shanghai 2024 Science and Technology Innovation Action Plan","the National Key R&D Program of China","the Agriculture Science and Technology Major Project of the ministry of Agriculture and Rural Affairs of China","Biological Breeding-Major Projects of the ministry of Agriculture and Rural Affairs of China","Science and Technology Major Project of Anhui Province","the National Key R&D Program of China","National Key Research and Development Program of China","NSFC grant","Program of Beijing Institute for Stem Cell and Regenerative Medicine","the \"Rising-Star Program\" of \"Shanghai 2024 Science and Technology Innovation Action Plan"],"pagination":["39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12908284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(1)"],"pubmed_abstract":["Background
As the ancestor of CRISPR-Cas12 nucleases, TnpB represents the most compact gene editing tool currently available. Recent studies have identified multiple TnpB systems with gene editing activity in mammalian cells, and the potential of TnpB in treating diseases has been demonstrated in animal models. However, the editing characteristics of various TnpB systems, comparable to CRISPR tools, require more extensive investigation.Results
Using a standardized evaluation framework, we conduct a thorough analysis of the editing properties of four TnpB variants alongside representative Cas12 and Cas9 tools applications. Overall, TnpBs exhibit intermediate editing activity and safety profiles among all tested systems, with ISYmu1 TnpB demonstrating a good performance in bo"],"journal":["Genome biology"],"pubmed_title":["Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing."],"pmcid":["PMC12908284"],"funding_grant_id":["202423110050063","2023ZD04074","24YF2703900","2022YFC3400201","2022FH122","31771485","2024YFA0917301","32101204"],"pubmed_authors":["Yuan S","Hong J","Xin C","Xiang G","Wan X","Wang H","Liu X","Hu J","Huo Y","Cao S","Wang Y","Liu D","Sun J"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.","description":"Background
As the ancestor of CRISPR-Cas12 nucleases, TnpB represents the most compact gene editing tool currently available. Recent studies have identified multiple TnpB systems with gene editing activity in mammalian cells, and the potential of TnpB in treating diseases has been demonstrated in animal models. However, the editing characteristics of various TnpB systems, comparable to CRISPR tools, require more extensive investigation.Results
Using a standardized evaluation framework, we conduct a thorough analysis of the editing properties of four TnpB variants alongside representative Cas12 and Cas9 tools applications. Overall, TnpBs exhibit intermediate editing activity and safety profiles among all tested systems, with ISYmu1 TnpB demonstrating a good performance in bo","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-15T22:12:21.018Z","creation":"2026-07-09T10:20:57.894Z"},"accession":"S-EPMC12908284","cross_references":{"pubmed":["41566394"],"doi":["10.1186/s13059-026-03949-8"]}}