{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Hanff V"],"pubmed_abstract":["In this work, we present several visible-light cleavable crRNAs targeting two different genes for regulating Cas9 activity. The crRNAs contain a total of three different photocleavable linker (PL) which induce a bond break when irradiated with a suitable wavelength. Cleavage of the crRNAs efficiently inhibits Cas9 activity, as demonstrated by the <i>in vitro</i> Cas9 assay developed in this work. By using visible light, our approach will enable precise downregulation of Cas9 activity in terms of location and timing, thereby enabling non-invasive and harm free control over genome editing for safer application."],"journal":["RSC chemical biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12908655"],"repository":["biostudies-literature"],"pubmed_title":["Optical control of Cas9 activity through visible-light cleavable crRNAs."],"pmcid":["PMC12908655"],"pubmed_authors":["Heckel A","Kaufmann J","Klimek R","Kim J","Langer KA","Jerabek S","Hanff V","Egli D"],"additional_accession":[]},"is_claimable":false,"name":"Optical control of Cas9 activity through visible-light cleavable crRNAs.","description":"In this work, we present several visible-light cleavable crRNAs targeting two different genes for regulating Cas9 activity. The crRNAs contain a total of three different photocleavable linker (PL) which induce a bond break when irradiated with a suitable wavelength. Cleavage of the crRNAs efficiently inhibits Cas9 activity, as demonstrated by the <i>in vitro</i> Cas9 assay developed in this work. By using visible light, our approach will enable precise downregulation of Cas9 activity in terms of location and timing, thereby enabling non-invasive and harm free control over genome editing for safer application.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-16T05:12:48.089Z","creation":"2026-07-09T10:37:34.625Z"},"accession":"S-EPMC12908655","cross_references":{"pubmed":["41704321"],"doi":["10.1039/d5cb00294j"]}}