biostudies-literatureGuo X| Postdoctoral Research Foundation of China (China Postdoctoral Research Foundation)Key Research and Development Program of Zhejiang Province中 国 博 士 后 科 学 基 金| Postdoctoral Research Foundation of Chinathe National Natural Science Foundation of ChinaKey Research and Development Program of Zhejiang Province (Key R&D plan of Zhejiang Province)1245-1263https://www.ebi.ac.uk/biostudies/studies/S-EPMC12909896biostudies-literatureUnknown45(4)Respiratory burst oxidase homolog D (RBOHD)-dependent reactive oxygen species (ROS) in Arabidopsis are well known to suppress pathogen colonization, but their influence on beneficial microbes remains unclear. Here, we found that the beneficial rhizobacterium Pseudomonas anguilliseptica was significantly less enriched in the rhizosphere of rbohD mutants than in that of wild-type plants. Conversely, elevated rhizosphere ROS levels, either triggered by pretreatment with pathogenic Dickeya solani bacteria or caused by mutations in ROS scavenging genes (e.g., in apx1 and cat2 mutants), promoted the rhizosphere recruitment of P. anguilliseptica. This promoting effect was abolished by catalase treatment. In situ microfluidic chemotaxis assays further revealed that P. anguilliseptica exhibits a chThe EMBO journalReactive oxygen species in the rhizosphere orchestrate the recruitment of beneficial bacteria.PMC129098962024M76285442090060,422772832024SSYS0104,2021C02064-7 and 2024ZD1000603Peng YLi JLi DGuo XLv XLiang YLi QLu LMa BHuang ZQi FSu YWang YDai HJia ZfalseReactive oxygen species in the rhizosphere orchestrate the recruitment of beneficial bacteria.Respiratory burst oxidase homolog D (RBOHD)-dependent reactive oxygen species (ROS) in Arabidopsis are well known to suppress pathogen colonization, but their influence on beneficial microbes remains unclear. Here, we found that the beneficial rhizobacterium Pseudomonas anguilliseptica was significantly less enriched in the rhizosphere of rbohD mutants than in that of wild-type plants. Conversely, elevated rhizosphere ROS levels, either triggered by pretreatment with pathogenic Dickeya solani bacteria or caused by mutations in ROS scavenging genes (e.g., in apx1 and cat2 mutants), promoted the rhizosphere recruitment of P. anguilliseptica. This promoting effect was abolished by catalase treatment. In situ microfluidic chemotaxis assays further revealed that P. anguilliseptica exhibits a ch2026-01-01T00:00:00Z2026 Feb2026-07-16T00:49:14.772Z2026-07-09T13:09:52.741ZS-EPMC129098964151414710.1038/s44318-025-00685-w