{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen C"],"funding":["Natural Science Foundation of Fujian Province"],"pagination":["41"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12911173"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(1)"],"pubmed_abstract":["<h4>Background</h4>The dynamic change of N6-methyladenosine (m<sup>6</sup>A) modification on substrate RNA molecules plays a critical role in different biological processes and disease pathogenesis. Although the beneficial effects of exercise training (ET) on skeletal muscle insulin resistance (IR) are well-established, the contribution of RNA m<sup>6</sup>A modification in ET-related adaptations in high-fat diet (HFD)-induced IR remains unclear.<h4>Results</h4>In this study, we show that exercise stimulation triggers a dynamic shift in skeletal muscle m<sup>6</sup>A modification levels during HFD consumption. As a key m<sup>6</sup>A methyltransferase, METTL16 was downregulated in HFD-fed mice and upregulated by ET at both the mRNA and protein levels. In vitro, METTL16 knockdown disrupted "],"journal":["BMC biology"],"pubmed_title":["METTL16-m&lt;sup&gt;6&lt;/sup&gt;A-PGC-1α axis contributes to exercise-induced mitochondrial adaptations in skeletal muscle of high-fat diet-fed insulin-resistant mice."],"pmcid":["PMC12911173"],"funding_grant_id":["2025J01959"],"pubmed_authors":["Xiang Q","Hou M","Huang C","Hu Y","Han X","Jiang C","Chen C","Zhou H","Wu H","Xu Y","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"METTL16-m&lt;sup&gt;6&lt;/sup&gt;A-PGC-1α axis contributes to exercise-induced mitochondrial adaptations in skeletal muscle of high-fat diet-fed insulin-resistant mice.","description":"<h4>Background</h4>The dynamic change of N6-methyladenosine (m<sup>6</sup>A) modification on substrate RNA molecules plays a critical role in different biological processes and disease pathogenesis. Although the beneficial effects of exercise training (ET) on skeletal muscle insulin resistance (IR) are well-established, the contribution of RNA m<sup>6</sup>A modification in ET-related adaptations in high-fat diet (HFD)-induced IR remains unclear.<h4>Results</h4>In this study, we show that exercise stimulation triggers a dynamic shift in skeletal muscle m<sup>6</sup>A modification levels during HFD consumption. As a key m<sup>6</sup>A methyltransferase, METTL16 was downregulated in HFD-fed mice and upregulated by ET at both the mRNA and protein levels. In vitro, METTL16 knockdown disrupted ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-16T00:50:01.654Z","creation":"2026-07-09T13:10:05.133Z"},"accession":"S-EPMC12911173","cross_references":{"pubmed":["41572276"],"doi":["10.1186/s12915-026-02519-5"]}}