{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang M"],"funding":["The National Natural Science Foundation of China"],"pagination":["44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12911376"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(1)"],"pubmed_abstract":["<h4>Background</h4>Dysregulation of N6-methyladenosine (m6A) has been implicated in the pathophysiology of various autoimmune diseases. However, its role in dermatomyositis (DM), particularly in cases associated with anti-MDA5 antibodies, remains unclear. This study aimed to elucidate the potential involvement of m6A modifications of mRNAs in the pathogenesis of anti-MDA5<sup>+</sup>DM.<h4>Methods</h4>We assessed mRNA m6A methylation levels in peripheral blood mononuclear cells (PBMCs) from anti-MDA5<sup>+</sup>DM patients and healthy controls (HC) using LC-MS/MS assay. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting were conducted to determine the mRNA and protein expression levels of YTHDF2 in PBMCs and monocytes from anti-MDA5<sup>+</sup>DM pa"],"journal":["Arthritis research & therapy"],"pubmed_title":["Dysregulation of YTHDF2 promotes the over expression of interferon beta in anti-MDA5 antibody-positive dermatomyositis."],"pmcid":["PMC12911376"],"funding_grant_id":["82572056, 82171788, 82371810"],"pubmed_authors":["Geng Q","Cao X","Lin S","Jin Q","Peng Q","Zhang M","Wang X","Jiao Y","Xiao C"],"additional_accession":[]},"is_claimable":false,"name":"Dysregulation of YTHDF2 promotes the over expression of interferon beta in anti-MDA5 antibody-positive dermatomyositis.","description":"<h4>Background</h4>Dysregulation of N6-methyladenosine (m6A) has been implicated in the pathophysiology of various autoimmune diseases. However, its role in dermatomyositis (DM), particularly in cases associated with anti-MDA5 antibodies, remains unclear. This study aimed to elucidate the potential involvement of m6A modifications of mRNAs in the pathogenesis of anti-MDA5<sup>+</sup>DM.<h4>Methods</h4>We assessed mRNA m6A methylation levels in peripheral blood mononuclear cells (PBMCs) from anti-MDA5<sup>+</sup>DM patients and healthy controls (HC) using LC-MS/MS assay. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting were conducted to determine the mRNA and protein expression levels of YTHDF2 in PBMCs and monocytes from anti-MDA5<sup>+</sup>DM pa","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-16T00:47:41.59Z","creation":"2026-07-09T10:26:31.966Z"},"accession":"S-EPMC12911376","cross_references":{"pubmed":["41580740"],"doi":["10.1186/s13075-026-03752-7"]}}