biostudies-literatureZhang MThe National Natural Science Foundation of China44https://www.ebi.ac.uk/biostudies/studies/S-EPMC12911376biostudies-literatureUnknown28(1)<h4>Background</h4>Dysregulation of N6-methyladenosine (m6A) has been implicated in the pathophysiology of various autoimmune diseases. However, its role in dermatomyositis (DM), particularly in cases associated with anti-MDA5 antibodies, remains unclear. This study aimed to elucidate the potential involvement of m6A modifications of mRNAs in the pathogenesis of anti-MDA5⁺DM.<h4>Methods</h4>We assessed mRNA m6A methylation levels in peripheral blood mononuclear cells (PBMCs) from anti-MDA5⁺DM patients and healthy controls (HC) using LC-MS/MS assay. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting were conducted to determine the mRNA and protein expression levels of YTHDF2 in PBMCs and monocytes from anti-MDA5⁺DM paArthritis research & therapyDysregulation of YTHDF2 promotes the over expression of interferon beta in anti-MDA5 antibody-positive dermatomyositis.PMC1291137682572056, 82171788, 82371810Geng QCao XLin SJin QPeng QZhang MWang XJiao YXiao CfalseDysregulation of YTHDF2 promotes the over expression of interferon beta in anti-MDA5 antibody-positive dermatomyositis.<h4>Background</h4>Dysregulation of N6-methyladenosine (m6A) has been implicated in the pathophysiology of various autoimmune diseases. However, its role in dermatomyositis (DM), particularly in cases associated with anti-MDA5 antibodies, remains unclear. This study aimed to elucidate the potential involvement of m6A modifications of mRNAs in the pathogenesis of anti-MDA5⁺DM.<h4>Methods</h4>We assessed mRNA m6A methylation levels in peripheral blood mononuclear cells (PBMCs) from anti-MDA5⁺DM patients and healthy controls (HC) using LC-MS/MS assay. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting were conducted to determine the mRNA and protein expression levels of YTHDF2 in PBMCs and monocytes from anti-MDA5⁺DM pa2026-01-01T00:00:00Z2026 Jan2026-07-16T00:47:41.59Z2026-07-09T10:26:31.966ZS-EPMC129113764158074010.1186/s13075-026-03752-7