biostudies-literatureWang WNanjing Medical University Fan Daiming Research Funds for Holistic Integrative MedicineNational Key Research and Development Program of China289-301https://www.ebi.ac.uk/biostudies/studies/S-EPMC12911655biostudies-literatureUnknown75(2)<h4>Background</h4>Current microbiome-based therapeutics face two prominent issues: the limited clinical efficacy of probiotics and the significant variability in the efficacy of microbiota transplantation across different diseases. Although washed microbiota transplantation (WMT) is a new faecal microbiota transplantation, a single therapeutic agent cannot be universally effective for multiple dysbiosis-related diseases.<h4>Objective</h4>We introduced a new therapeutic concept, X-augmented WMT (X-auWMT), which combines a disease-specific beneficial microbe, 'X', with WMT to enhance its effectiveness. Our goal was to identify a candidate 'X' bacterium to augment WMT efficacy and examine the efficacy of X-auWMT in animal models of radiation enteritis (RE).<h4>Design</h4>We conducted a prospGut&lt;i&gt;Holdemanella biformis&lt;/i&gt; augments washed microbiota transplantation for the treatment of radiation enteritis.PMC129116552023-3HIM2021YFA0717004Zhang FLu CWen QLi PZhang LZhang SLiang CDing XLu GWang RYi BWang WBai JWang YYu YCui Bfalse&lt;i&gt;Holdemanella biformis&lt;/i&gt; augments washed microbiota transplantation for the treatment of radiation enteritis.<h4>Background</h4>Current microbiome-based therapeutics face two prominent issues: the limited clinical efficacy of probiotics and the significant variability in the efficacy of microbiota transplantation across different diseases. Although washed microbiota transplantation (WMT) is a new faecal microbiota transplantation, a single therapeutic agent cannot be universally effective for multiple dysbiosis-related diseases.<h4>Objective</h4>We introduced a new therapeutic concept, X-augmented WMT (X-auWMT), which combines a disease-specific beneficial microbe, 'X', with WMT to enhance its effectiveness. Our goal was to identify a candidate 'X' bacterium to augment WMT efficacy and examine the efficacy of X-auWMT in animal models of radiation enteritis (RE).<h4>Design</h4>We conducted a prosp2026-01-01T00:00:00Z2026 Jan2026-07-16T07:11:47.242Z2026-07-09T10:43:13.497ZS-EPMC129116554094713810.1136/gutjnl-2025-335230