{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Soler-Oliva ME"],"funding":["Ministerio de Ciencia, Innovación y Universidades","European Research Council","Fundación de Investigación de la Universidad de Sevilla","Universidad de Sevilla","Agencia Estatal de Investigación"],"pagination":["e1012040"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12912698"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(2)"],"pubmed_abstract":["Transcription is essential for cellular function, but it can also lead to genetic instability, particularly through the formation of secondary structures such as R-loops, which consist of an RNA-DNA hybrid and a displaced DNA strand. Unscheduled R-loop accumulation is a major source of DNA damage and has been associated with several human diseases, including cancer. While multiple factors involved in RNA biogenesis, export, and chromatin remodeling play a role in preventing R-loop accumulation, the function of essential proteins in R-loop metabolism remains unexplored. Here, we performed a genetic screening in Saccharomyces cerevisiae using over 1200 temperature-sensitive mutants to identify novel proteins involved in the prevention of R-loop-associated genomic instability. Our results rev"],"journal":["PLoS genetics"],"pubmed_title":["An essential gene screening identifies yeast Mot1 as a suppressor of R-loops and genome instability."],"pmcid":["PMC12912698"],"funding_grant_id":["PID2019-104270GB-I00/BMC","VII PPIT-US","PID2022-138251NB-I00","ERC2014 AdG669898 TARLOOP","FIUS22/0178","PID2022-140466NB-I00"],"pubmed_authors":["Dominguez-Sierra RA","Aguilera A","Soler-Oliva ME","Gaillard H"],"additional_accession":[]},"is_claimable":false,"name":"An essential gene screening identifies yeast Mot1 as a suppressor of R-loops and genome instability.","description":"Transcription is essential for cellular function, but it can also lead to genetic instability, particularly through the formation of secondary structures such as R-loops, which consist of an RNA-DNA hybrid and a displaced DNA strand. Unscheduled R-loop accumulation is a major source of DNA damage and has been associated with several human diseases, including cancer. While multiple factors involved in RNA biogenesis, export, and chromatin remodeling play a role in preventing R-loop accumulation, the function of essential proteins in R-loop metabolism remains unexplored. Here, we performed a genetic screening in Saccharomyces cerevisiae using over 1200 temperature-sensitive mutants to identify novel proteins involved in the prevention of R-loop-associated genomic instability. Our results rev","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T00:37:11.409Z","creation":"2026-07-09T10:26:11.115Z"},"accession":"S-EPMC12912698","cross_references":{"pubmed":["41662397"],"doi":["10.1371/journal.pgen.1012040"]}}