biostudies-literatureUnknownDeshpande TCore unit PIX of the Medical Faculty and the IZKF MünsterEuropean Research Councildeutsche forschungsgemeinschaftbundesministerium für bildung und forschungWe investigated whether basement membrane (BM) laminins influence regional differences in the vasculature by performing single-cell RNA sequencing on cerebral blood vessels from mice lacking the major vascular laminins in endothelial and smooth muscle BMs, laminin α4 (<i>Lama4</i>^{<i>^-/-</i>} ) and laminin α5 (<i>Tek-cre:Lama5</i>^{<i>^-/-</i>}), and wild-type littermates. Our dataset expands existing cerebral vascular transcriptomic profiles and reveals that <i>Lama4</i>^{^<i>-/-</i>} endothelial cells exhibit increased arterial marker expression and reduced postcapillary venule identity. In vitro and in vivo studies indicated that compensatory upregulation of laminin α5 in <i>Lama4</i>^{<i>^-/-</i>} vessels enhanceJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism271678X251410040https://www.ebi.ac.uk/biostudies/studies/S-EPMC12913041biostudies-literatureVascular basement membrane laminins modulate functional zonation of cerebral microvessels.PMC12913041SO285/14-1DeCoDis101054805Huppert JHannocks MJSamawar SKAdams RHGhavampour SSong JJeong HWDeshpande TSorokin LThulichery DRSoltwisch JBudny SFaber CMeng LWachsmuth LKapupara KHallmann RfalseVascular basement membrane laminins modulate functional zonation of cerebral microvessels.We investigated whether basement membrane (BM) laminins influence regional differences in the vasculature by performing single-cell RNA sequencing on cerebral blood vessels from mice lacking the major vascular laminins in endothelial and smooth muscle BMs, laminin α4 (<i>Lama4</i>^{<i>^-/-</i>} ) and laminin α5 (<i>Tek-cre:Lama5</i>^{<i>^-/-</i>}), and wild-type littermates. Our dataset expands existing cerebral vascular transcriptomic profiles and reveals that <i>Lama4</i>^{^<i>-/-</i>} endothelial cells exhibit increased arterial marker expression and reduced postcapillary venule identity. In vitro and in vivo studies indicated that compensatory upregulation of laminin α5 in <i>Lama4</i>^{<i>^-/-</i>} vessels enhance2026-01-01T00:00:00Z2026 Feb2026-07-16T06:52:07.974Z2026-07-09T10:42:18.342ZS-EPMC129130414169680610.1177/0271678X251410040