{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Laudisi F"],"funding":["Ministero dell'Università e della Ricerca"],"pagination":["18"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12914907"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(1)"],"pubmed_abstract":["<h4>Background and aims</h4>Defects of the gut epithelial barrier and counter-regulatory mechanisms are a prerequisite for the onset of intestinal inflammation in patients with inflammatory bowel diseases (IBD). Elevated levels of Smad7, an inhibitor of TGF-β1, in both epithelial and immune cells have been associated with the pathological processes observed in IBD. We aim to determine the relevance of Smad7 expression in the epithelial compartment.<h4>Methods</h4>We generated mice overexpressing Smad7 in the gut epithelium (Smad7TgCre<sup>+</sup>) and monitored pathology development. Inflammatory cell infiltration was assessed using multiplex immunofluorescence and flow cytometry. To investigate the molecular mechanisms underlying ileal damage, we performed spatial transcriptomics on froze"],"journal":["Journal of biomedical science"],"pubmed_title":["Intestinal epithelial Smad7 drives purine metabolic dysregulation and ileal inflammation."],"pmcid":["PMC12914907"],"funding_grant_id":["PNRR M4C2I1.3 PE6 project PE00000019 Heal Italia","PRIN 2022JEBP88,"],"pubmed_authors":["Villablanca EJ","Stolfi C","Zorzi F","He N","Sica GS","Melaiu O","Monasterio G","Sommella EM","Fantini MC","Pacifico T","Monteleone I","Serra MA","Tomassini L","Ortenzi A","D'Oria V","Monteleone G","Laudisi F","De Stefanis C"],"additional_accession":[]},"is_claimable":false,"name":"Intestinal epithelial Smad7 drives purine metabolic dysregulation and ileal inflammation.","description":"<h4>Background and aims</h4>Defects of the gut epithelial barrier and counter-regulatory mechanisms are a prerequisite for the onset of intestinal inflammation in patients with inflammatory bowel diseases (IBD). Elevated levels of Smad7, an inhibitor of TGF-β1, in both epithelial and immune cells have been associated with the pathological processes observed in IBD. We aim to determine the relevance of Smad7 expression in the epithelial compartment.<h4>Methods</h4>We generated mice overexpressing Smad7 in the gut epithelium (Smad7TgCre<sup>+</sup>) and monitored pathology development. Inflammatory cell infiltration was assessed using multiplex immunofluorescence and flow cytometry. To investigate the molecular mechanisms underlying ileal damage, we performed spatial transcriptomics on froze","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T06:30:55.512Z","creation":"2026-07-09T10:39:09.506Z"},"accession":"S-EPMC12914907","cross_references":{"pubmed":["41703574"],"doi":["10.1186/s12929-026-01224-3"]}}