{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu S"],"funding":["Graduate Education Quailty Enhancement Project of Anhui Province","National Natural Science Foundation of China","Joint Funds of the National Natural Science Foundation of China"],"pagination":["e0197125"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12915358"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["92(2)"],"pubmed_abstract":["Laccases are present as isozymes in white-rot fungi, yet their evolutionary history and functional role in lignin degradation remain controversial. <i>Trametes hirsuta</i>, a ubiquitous fungus in forest ecosystems, can completely break down cellulose, hemicellulose, and lignin in wood. Based on bioinformatic and biochemical characterization, we have shown that five laccase isozyme genes (<i>lacA-E</i>) in <i>Trametes hirsuta</i> AH28-2 were derived from a single ancestral laccase gene, <i>lacF</i>, with <i>lacA</i> and <i>lacB</i> originating from disparate evolutionary branches. The syringyl-type (S-type) lignin model compounds significantly induced the expression of <i>lacA-lacE</i> at both transcriptional and expression levels. Furthermore, <i>in vitro</i> and <i>in vivo</i> analyses de"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Adaptive evolution of Agaricomycetes laccases follows wood lignin diversification in plants."],"pmcid":["PMC12915358"],"funding_grant_id":["U22A20442","32370133","2023xscx024","32470122"],"pubmed_authors":["Liu J","Fang Z","Yin T","Wang C","Yu Q","Zhang X","Zhou R","Xiao Y","Liu S"],"additional_accession":[]},"is_claimable":false,"name":"Adaptive evolution of Agaricomycetes laccases follows wood lignin diversification in plants.","description":"Laccases are present as isozymes in white-rot fungi, yet their evolutionary history and functional role in lignin degradation remain controversial. <i>Trametes hirsuta</i>, a ubiquitous fungus in forest ecosystems, can completely break down cellulose, hemicellulose, and lignin in wood. Based on bioinformatic and biochemical characterization, we have shown that five laccase isozyme genes (<i>lacA-E</i>) in <i>Trametes hirsuta</i> AH28-2 were derived from a single ancestral laccase gene, <i>lacF</i>, with <i>lacA</i> and <i>lacB</i> originating from disparate evolutionary branches. The syringyl-type (S-type) lignin model compounds significantly induced the expression of <i>lacA-lacE</i> at both transcriptional and expression levels. Furthermore, <i>in vitro</i> and <i>in vivo</i> analyses de","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T06:32:42.653Z","creation":"2026-07-09T10:39:33.92Z"},"accession":"S-EPMC12915358","cross_references":{"pubmed":["41528145"],"doi":["10.1128/aem.01971-25"]}}