{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Balouz V"],"funding":["CONICET","Agencia Nacional de Promoción CientÃfica y Tecnológica"],"pagination":["e0013728"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12915924"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(2)"],"pubmed_abstract":["<h4>Background</h4>Chagas disease (ChD), caused by the parasitic protozoan Trypanosoma cruzi, is a lifelong, neglected tropical disease with substantial medical and socioeconomic impact. Despite this situation, currently available diagnostic and therapeutic methods display serious limitations. A promising strategy to improve ChD serodiagnosis involves targeting parasite carbohydrate antigens, particularly the α-galactosyl-rich mucins that coat the surface of bloodstream trypomastigotes (tGPI-mucins).<h4>Methods/principle findings</h4>Here, we present a concise and efficient protocol for the chemical synthesis of a tGPI-mucin-derived glycotope, the disaccharide α-D-Galp-(1 → 3)-β-D-Galp, and its functional conjugation to different scaffolds using the squarate method. A neoglycoprotein made "],"journal":["PLoS neglected tropical diseases"],"pubmed_title":["Conjugates of α-d-Galp-(1→3)-β-d-Galp for the serological diagnosis of Chagas disease."],"pmcid":["PMC12915924"],"funding_grant_id":["PICT-2021-I-A-00284","PICT-2021-I-A-00441"],"pubmed_authors":["Melli LJ","Altcheh J","Ducrey I","Abal M","Balouz V","Marino C","Lopez R","Buscaglia CA","de Lederkremer RM","Ciocchini AE","Conte N","Giorgi ME"],"additional_accession":[]},"is_claimable":false,"name":"Conjugates of α-d-Galp-(1→3)-β-d-Galp for the serological diagnosis of Chagas disease.","description":"<h4>Background</h4>Chagas disease (ChD), caused by the parasitic protozoan Trypanosoma cruzi, is a lifelong, neglected tropical disease with substantial medical and socioeconomic impact. Despite this situation, currently available diagnostic and therapeutic methods display serious limitations. A promising strategy to improve ChD serodiagnosis involves targeting parasite carbohydrate antigens, particularly the α-galactosyl-rich mucins that coat the surface of bloodstream trypomastigotes (tGPI-mucins).<h4>Methods/principle findings</h4>Here, we present a concise and efficient protocol for the chemical synthesis of a tGPI-mucin-derived glycotope, the disaccharide α-D-Galp-(1 → 3)-β-D-Galp, and its functional conjugation to different scaffolds using the squarate method. A neoglycoprotein made ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-09T11:05:15.708Z","creation":"2026-07-09T10:41:05.988Z"},"accession":"S-EPMC12915924","cross_references":{"pubmed":["41666228"],"doi":["10.1371/journal.pntd.0013728"]}}