biostudies-literatureBalouz VCONICETAgencia Nacional de Promoción CientÃfica y Tecnológicae0013728https://www.ebi.ac.uk/biostudies/studies/S-EPMC12915924biostudies-literatureUnknown20(2)<h4>Background</h4>Chagas disease (ChD), caused by the parasitic protozoan Trypanosoma cruzi, is a lifelong, neglected tropical disease with substantial medical and socioeconomic impact. Despite this situation, currently available diagnostic and therapeutic methods display serious limitations. A promising strategy to improve ChD serodiagnosis involves targeting parasite carbohydrate antigens, particularly the α-galactosyl-rich mucins that coat the surface of bloodstream trypomastigotes (tGPI-mucins).<h4>Methods/principle findings</h4>Here, we present a concise and efficient protocol for the chemical synthesis of a tGPI-mucin-derived glycotope, the disaccharide α-D-Galp-(1 → 3)-β-D-Galp, and its functional conjugation to different scaffolds using the squarate method. A neoglycoprotein made PLoS neglected tropical diseasesConjugates of α-d-Galp-(1→3)-β-d-Galp for the serological diagnosis of Chagas disease.PMC12915924PICT-2021-I-A-00284PICT-2021-I-A-00441Melli LJAltcheh JDucrey IAbal MBalouz VMarino CLopez RBuscaglia CAde Lederkremer RMCiocchini AEConte NGiorgi MEfalseConjugates of α-d-Galp-(1→3)-β-d-Galp for the serological diagnosis of Chagas disease.<h4>Background</h4>Chagas disease (ChD), caused by the parasitic protozoan Trypanosoma cruzi, is a lifelong, neglected tropical disease with substantial medical and socioeconomic impact. Despite this situation, currently available diagnostic and therapeutic methods display serious limitations. A promising strategy to improve ChD serodiagnosis involves targeting parasite carbohydrate antigens, particularly the α-galactosyl-rich mucins that coat the surface of bloodstream trypomastigotes (tGPI-mucins).<h4>Methods/principle findings</h4>Here, we present a concise and efficient protocol for the chemical synthesis of a tGPI-mucin-derived glycotope, the disaccharide α-D-Galp-(1 → 3)-β-D-Galp, and its functional conjugation to different scaffolds using the squarate method. A neoglycoprotein made 2026-01-01T00:00:00Z2026 Feb2026-07-09T11:05:15.708Z2026-07-09T10:41:05.988ZS-EPMC129159244166622810.1371/journal.pntd.0013728