biostudies-literatureLi SNIDDK NIH HHSNIA NIH HHSNCI NIH HHS2381-2394https://www.ebi.ac.uk/biostudies/studies/S-EPMC12916282biostudies-literatureUnknown57(10)Cellular senescence is a complex biological process that plays a pathophysiological role in aging and age-related diseases. The biological understanding of senescence at the cellular and tissue levels remains incomplete due to the lack of specific biomarkers as well as the relative rarity of senescent cells, their phenotypic heterogeneity and dynamic features. This Review provides a comprehensive overview of multiomic approaches for the characterization and biological understanding of cellular senescence. The technical capability and challenges of each approach are discussed, and practical guidelines are provided for selecting tools for identifying, characterizing and spatially mapping senescent cells. The importance of computational analyses in multiomics research, including senescent celNature geneticsAdvancing biological understanding of cellular senescence with computational multiomics.PMC12916282R01 DK132535U54 AG075932UH3 CA275681Konigshoff MGarovic VZhang KMitchell JTNagaraj SLu YNiedernhofer LJDong MSchmidt ELShu JRobson PChuang JHWang JMartini HSun LTchkonia TWang SXie JGu LKirkland JLBecich MJEnninful ALee JHRojas MXu MLi DWu PHChen JTeneche MGMora ALAdams PDRahman IVanegas NJeon HBenz CElisseeff JMelov SLi SDileepan MSenNet ConsortiumHu QSchafer MJRosas LMa QYu MHertzel AVBueno MMusi NPassos JFBaker DJGlass CLugo-Martinez JAliferis CZhao YDing LFranco ACFarzad NMenon VLiu YBernlohr DAFlynn WFLeBrasseur NKKummerfeld ESo PTCCalyeca JAguayo-Mazzucato CRamasamy RIwasaki KRosenthal NFertig EJRindone ANChilds BGTaha HBHalene SSengupta KYin SDuncan FRodriguez-Lopez JKang JWKarmakar SSchneider KFan RAgudelo Garcia PASchilling BChung DCosgrove BDMa ARobbins PDZhang XNelson ACKorstanje RHerman ABJi ZFurman DPeters STZhu QfalseAdvancing biological understanding of cellular senescence with computational multiomics.Cellular senescence is a complex biological process that plays a pathophysiological role in aging and age-related diseases. The biological understanding of senescence at the cellular and tissue levels remains incomplete due to the lack of specific biomarkers as well as the relative rarity of senescent cells, their phenotypic heterogeneity and dynamic features. This Review provides a comprehensive overview of multiomic approaches for the characterization and biological understanding of cellular senescence. The technical capability and challenges of each approach are discussed, and practical guidelines are provided for selecting tools for identifying, characterizing and spatially mapping senescent cells. The importance of computational analyses in multiomics research, including senescent cel2025-01-01T00:00:00Z2025 Oct2026-07-09T11:04:31.147Z2026-07-09T10:39:15.427ZS-EPMC129162824095424910.1038/s41588-025-02314-y