{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kulshreshtha A"],"funding":["Texas A&M AgriLife Research Insect-vectored Disease Seed Grants","Texas A&M AgriLife Institute for Advancing Health Through Agriculture","National Institute of Food and Agriculture","Texas A&M Vegetable and Fruit Improvement Center"],"pagination":["e70745"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12917295"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["125(4)"],"pubmed_abstract":["Huanglongbing (HLB) disease, associated with the fastidious bacterium Candidatus Liberibacter asiaticus (CLas), has a significant impact on citrus production worldwide. Conventional biochemical and genetic evaluation studies to identify potential disease resistance strategies have been mainly hindered due to the inability to culture CLas in a defined medium and the general recalcitrance of Citrus cultivars (grapefruits and oranges) to Agrobacterium-mediated plant transformation. We previously demonstrated the utility of plant hairy roots to co-cultivate CLas. In this study, we developed a hairy root transformation system using citron (Citrus medica L.), which is highly amenable to Rhizobium-mediated hairy root transformation. The explant survival and hairy root transformation efficiencies "],"journal":["The Plant journal : for cell and molecular biology"],"pubmed_title":["High-efficiency genome-editing, transgene evaluation, and antimicrobial efficacy testing using Citrus medica L. hairy roots."],"pmcid":["PMC12917295"],"funding_grant_id":["2021-70029-36056","2025-70029-44033","2019-70016-29066","124190-96210","2024-51181-43464","TEX0-7790","HATCH TEX09621"],"pubmed_authors":["Ramasamy M","Tu CK","Kulshreshtha A","Irigoyen S","Laughlin K","Mandadi KK","Padilla CS","Borneman J"],"additional_accession":[]},"is_claimable":false,"name":"High-efficiency genome-editing, transgene evaluation, and antimicrobial efficacy testing using Citrus medica L. hairy roots.","description":"Huanglongbing (HLB) disease, associated with the fastidious bacterium Candidatus Liberibacter asiaticus (CLas), has a significant impact on citrus production worldwide. Conventional biochemical and genetic evaluation studies to identify potential disease resistance strategies have been mainly hindered due to the inability to culture CLas in a defined medium and the general recalcitrance of Citrus cultivars (grapefruits and oranges) to Agrobacterium-mediated plant transformation. We previously demonstrated the utility of plant hairy roots to co-cultivate CLas. In this study, we developed a hairy root transformation system using citron (Citrus medica L.), which is highly amenable to Rhizobium-mediated hairy root transformation. The explant survival and hairy root transformation efficiencies ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T10:08:54.102Z","creation":"2026-07-09T10:48:19.142Z"},"accession":"S-EPMC12917295","cross_references":{"pubmed":["41709520"],"doi":["10.1111/tpj.70745"]}}