biostudies-literatureKulshreshtha ATexas A&M AgriLife Research Insect-vectored Disease Seed GrantsTexas A&M AgriLife Institute for Advancing Health Through AgricultureNational Institute of Food and AgricultureTexas A&M Vegetable and Fruit Improvement Centere70745https://www.ebi.ac.uk/biostudies/studies/S-EPMC12917295biostudies-literatureUnknown125(4)Huanglongbing (HLB) disease, associated with the fastidious bacterium Candidatus Liberibacter asiaticus (CLas), has a significant impact on citrus production worldwide. Conventional biochemical and genetic evaluation studies to identify potential disease resistance strategies have been mainly hindered due to the inability to culture CLas in a defined medium and the general recalcitrance of Citrus cultivars (grapefruits and oranges) to Agrobacterium-mediated plant transformation. We previously demonstrated the utility of plant hairy roots to co-cultivate CLas. In this study, we developed a hairy root transformation system using citron (Citrus medica L.), which is highly amenable to Rhizobium-mediated hairy root transformation. The explant survival and hairy root transformation efficiencies The Plant journal : for cell and molecular biologyHigh-efficiency genome-editing, transgene evaluation, and antimicrobial efficacy testing using Citrus medica L. hairy roots.PMC129172952021-70029-360562025-70029-440332019-70016-29066124190-962102024-51181-43464TEX0-7790HATCH TEX09621Ramasamy MTu CKKulshreshtha AIrigoyen SLaughlin KMandadi KKPadilla CSBorneman JfalseHigh-efficiency genome-editing, transgene evaluation, and antimicrobial efficacy testing using Citrus medica L. hairy roots.Huanglongbing (HLB) disease, associated with the fastidious bacterium Candidatus Liberibacter asiaticus (CLas), has a significant impact on citrus production worldwide. Conventional biochemical and genetic evaluation studies to identify potential disease resistance strategies have been mainly hindered due to the inability to culture CLas in a defined medium and the general recalcitrance of Citrus cultivars (grapefruits and oranges) to Agrobacterium-mediated plant transformation. We previously demonstrated the utility of plant hairy roots to co-cultivate CLas. In this study, we developed a hairy root transformation system using citron (Citrus medica L.), which is highly amenable to Rhizobium-mediated hairy root transformation. The explant survival and hairy root transformation efficiencies 2026-01-01T00:00:00Z2026 Feb2026-07-16T10:08:54.102Z2026-07-09T10:48:19.142ZS-EPMC129172954170952010.1111/tpj.70745