biostudies-literatureUnknown10(4)Ferrant J<h4>Abstract</h4>Diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) are the 2 most common B-cell lymphomas and are characterized by a dynamic crosstalk between tumor B cells and a heterogeneous, tumor-supportive microenvironment, such as immune, endothelial, and stromal components. Despite their recognized impact on the pathogenesis and prognosis of B-cell lymphoma, tumor-associated macrophages (TAM) have not been extensively explored in these diseases. In this study, we investigated mononuclear phagocyte (MNP) heterogeneity at the single-cell level and the activation profile of MNP, stromal, and endothelial compartments, in B-cell lymphoma lymph nodes compared to reactive secondary lymphoid organs. This was achieved using a combination of mass cytometry, single-cell RNA seBlood advances1217-1232https://www.ebi.ac.uk/biostudies/studies/S-EPMC12917531biostudies-literatureMyeloid landscape profiling identifies DLBCL-specific suppressive macrophages colocalized with blood endothelial cells.PMC12917531Hoareau BMichaud HALaunay VRoussel MPapa IPangault CVan Acker NManson GDeleurme LMonvoisin CPadonou FAlbaud BLlamas Gutierrez FTarte KLeonard SBonnefoy NFerrant JShipp MAPecot TDubec-Fleury CLe Gallou SBarthel ALaurent CBrottier CfalseMyeloid landscape profiling identifies DLBCL-specific suppressive macrophages colocalized with blood endothelial cells.<h4>Abstract</h4>Diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) are the 2 most common B-cell lymphomas and are characterized by a dynamic crosstalk between tumor B cells and a heterogeneous, tumor-supportive microenvironment, such as immune, endothelial, and stromal components. Despite their recognized impact on the pathogenesis and prognosis of B-cell lymphoma, tumor-associated macrophages (TAM) have not been extensively explored in these diseases. In this study, we investigated mononuclear phagocyte (MNP) heterogeneity at the single-cell level and the activation profile of MNP, stromal, and endothelial compartments, in B-cell lymphoma lymph nodes compared to reactive secondary lymphoid organs. This was achieved using a combination of mass cytometry, single-cell RNA se2026-01-01T00:00:00Z2026 Feb2026-07-16T09:17:52.444Z2026-07-09T10:47:00.089ZS-EPMC129175314106115410.1182/bloodadvances.2024015689