biostudies-literatureCadenas-Jimenez ICentro de Investigación Biomédica en Red de Enfermedades InfecciosasFondo de Investigaciones SanitariasSociedad Española de Neumología y Cirugía TorácicaCentro de Investigación Biomédica en Red de Enfermedades Respiratorias87https://www.ebi.ac.uk/biostudies/studies/S-EPMC12918164biostudies-literatureUnknown27(1)<h4>Background</h4>Bronchiectasis is a chronic respiratory disease characterized by recurrent exacerbations and persistent inflammation, often associated with bacterial pathogens such as Haemophilus influenzae and Pseudomonas aeruginosa. Phenotypic adaptations (e.g., antimicrobial resistance) complicate treatment and worsen a patient's quality of life.<h4>Methods</h4>Between 2019 and 2020, we isolated 52 H. influenzae and 48 P. aeruginosa strains from 62 non-CF bronchiectasis patients across three scheduled visits and during exacerbation episodes. Antimicrobial susceptibility (assessed by microdilution) and phenotyping assays (motility and hypermutability) were performed. Whole genome sequencing was applied for analyses of resistance determinants, virulence factors, and genetic diversity.<Respiratory researchEpidemiological insights into Haemophilus influenzae and Pseudomonas aeruginosa persistent colonization in non-cystic fibrosis bronchiectasis patients: a longitudinal and multicenter study.PMC12918164698/2018CB06/06/1089PI22/00257CB21/13/00009CB06/06/0037Rodriguez LOliver ASantos SMillares LVendrell MSimon MArdanuy CDominguez MABenaali-Bakkar YCasabella ACalvo-Silveria SMonton CGoncalves-Carvalho FMunoz GSaiz-Escobedo LQuero SAntuori AArque ECadenas-Jimenez ICamps-Massa PGonzalez-Diaz ANavarro ALacoma ABronchiomics groupMarti SSuarez-Cuartin GBarreiro EPolverino ECalero CPlasencia VLlunell AGarcia-Olive IPrat-Aymerich CMarin ACuchi ETubau FPomares JfalseEpidemiological insights into Haemophilus influenzae and Pseudomonas aeruginosa persistent colonization in non-cystic fibrosis bronchiectasis patients: a longitudinal and multicenter study.<h4>Background</h4>Bronchiectasis is a chronic respiratory disease characterized by recurrent exacerbations and persistent inflammation, often associated with bacterial pathogens such as Haemophilus influenzae and Pseudomonas aeruginosa. Phenotypic adaptations (e.g., antimicrobial resistance) complicate treatment and worsen a patient's quality of life.<h4>Methods</h4>Between 2019 and 2020, we isolated 52 H. influenzae and 48 P. aeruginosa strains from 62 non-CF bronchiectasis patients across three scheduled visits and during exacerbation episodes. Antimicrobial susceptibility (assessed by microdilution) and phenotyping assays (motility and hypermutability) were performed. Whole genome sequencing was applied for analyses of resistance determinants, virulence factors, and genetic diversity.<2026-01-01T00:00:00Z2026 Feb2026-07-09T11:25:12.054Z2026-07-09T10:48:12.851ZS-EPMC129181644169969510.1186/s12931-026-03553-1