{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Alawar N"],"funding":["European Research Council","Deutsche Forschungsgemeinschaft"],"pagination":["e70239"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12919372"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(2)"],"pubmed_abstract":["Small extracellular vesicles (sEVs) derived from cytotoxic T lymphocytes (CTLs) are emerging as potential mediators of antitumor immunity; however, their subcellular origins and functional properties remain incompletely defined. In this study, we investigated the intracellular routes and cytotoxic potential of CTL-derived exosomes. Using correlative light and electron microscopy, we discovered that CTL-derived exosomes originate from both classical multivesicular bodies (MVBs) and the recently identified multi core granules (MCGs). Through total internal reflection fluorescence microscopy, we demonstrated that, in contrast to MVB-derived exosomes, MCG-derived exosomes are released at the immunological synapse in a stimulus-dependent manner. To enable functional characterization, we develop"],"journal":["Journal of extracellular vesicles"],"pubmed_title":["T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies."],"pmcid":["PMC12919372"],"funding_grant_id":["ERC-2021-SyG_951329","ID number 469256325","ID number 203827099","SFB 894","ID number 157660137"],"pubmed_authors":["Rettig J","Keller A","Schirra C","Dustin ML","Fecher-Trost C","Meyer MR","Flockerzi V","Rasuli R","Becherer U","Alawar N","Weins L"],"additional_accession":[]},"is_claimable":false,"name":"T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies.","description":"Small extracellular vesicles (sEVs) derived from cytotoxic T lymphocytes (CTLs) are emerging as potential mediators of antitumor immunity; however, their subcellular origins and functional properties remain incompletely defined. In this study, we investigated the intracellular routes and cytotoxic potential of CTL-derived exosomes. Using correlative light and electron microscopy, we discovered that CTL-derived exosomes originate from both classical multivesicular bodies (MVBs) and the recently identified multi core granules (MCGs). Through total internal reflection fluorescence microscopy, we demonstrated that, in contrast to MVB-derived exosomes, MCG-derived exosomes are released at the immunological synapse in a stimulus-dependent manner. To enable functional characterization, we develop","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T11:55:15.277Z","creation":"2026-07-09T10:52:01.401Z"},"accession":"S-EPMC12919372","cross_references":{"pubmed":["41711633"],"doi":["10.1002/jev2.70239"]}}