biostudies-literatureLeineweber WDNIH National Institute of General Medical SciencesSchmidt FuturesNational Institutes of HealthNIGMS NIH HHS151492https://www.ebi.ac.uk/biostudies/studies/S-EPMC12919655biostudies-literatureUnknown104(2)Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization.European journal of cell biologyHolotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization.PMC12919655SC2 GM140991SC2GM140991Michael RLeineweber WDNoyer JLeycam CMunares GAJurney PfalseHolotomographic microscopy reveals label-free quantitative dynamics of endothelial cells during endothelialization.Holotomograhic microscopy (HTM) has emerged as a non-invasive imaging technique that offers high-resolution, quantitative 3D imaging of biological samples. This study explores the application of HTM in examining endothelial cells (ECs). HTM overcomes the limitations of traditional microscopy methods in capturing the real-time dynamics of ECs by leveraging the refractive index (RI) to map 3D distributions label-free. This work demonstrates the utility of HTM in visualizing key cellular processes during endothelialization, wherein ECs anchor, adhere, migrate, and proliferate. Leveraging the high resolution and quantitative power of HTM, we show that lipid droplets and mitochondria are readily visualized, enabling more comprehensive studies on their respective roles during endothelialization.2025-01-01T00:00:00Z2025 Jun2026-07-09T11:23:23.188Z2026-07-09T10:46:37.423ZS-EPMC129196554030050110.1016/j.ejcb.2025.151492