biostudies-literatureUnknown20(2)Doering JEModernaBreast milk confers infants with immunity to a multitude of pathogens reflective of prior maternal infections and vaccinations. However, in outbreak situations where infants may be vulnerable to lethal infections due to gaps in the maternal immune repertoire, a case can be made for supplementing breast milk with one or more pathogen-specific monoclonal antibodies (mAbs) with known prophylactic or therapeutic activity. As oral delivery of recombinant IgG and IgA mAbs to infants has proven challenging, we investigated the use of mRNA-lipid nanoparticle (LNP) technology to stimulate pathogen-specific mAbs in milk. mRNA encoding the Vibrio cholerae O1 specific mAb, ZAC-3, as a human IgG1 or dimeric IgA2, was encapsulated in lipid nanoparticles (LNP) and administered parenterally to lactating aPLoS neglected tropical diseasese0013518https://www.ebi.ac.uk/biostudies/studies/S-EPMC12919922biostudies-literatureMaternal expression and breast milk transfer of an mRNA- encoded monoclonal antibody in a murine model of cholera.PMC12919922Plante ODoering JECarfi AAdewunmi YMantis NJDeal CEfalseMaternal expression and breast milk transfer of an mRNA- encoded monoclonal antibody in a murine model of cholera.Breast milk confers infants with immunity to a multitude of pathogens reflective of prior maternal infections and vaccinations. However, in outbreak situations where infants may be vulnerable to lethal infections due to gaps in the maternal immune repertoire, a case can be made for supplementing breast milk with one or more pathogen-specific monoclonal antibodies (mAbs) with known prophylactic or therapeutic activity. As oral delivery of recombinant IgG and IgA mAbs to infants has proven challenging, we investigated the use of mRNA-lipid nanoparticle (LNP) technology to stimulate pathogen-specific mAbs in milk. mRNA encoding the Vibrio cholerae O1 specific mAb, ZAC-3, as a human IgG1 or dimeric IgA2, was encapsulated in lipid nanoparticles (LNP) and administered parenterally to lactating a2026-01-01T00:00:00Z2026 Feb2026-07-16T09:24:24.593Z2026-07-09T10:46:47.207ZS-EPMC129199224166241210.1371/journal.pntd.0013518