biostudies-literatureUnknown17Kelm M<h4>Introduction</h4>Blinatumomab (BLN) is a bispecific T-cell engager that has revolutionized the treatment of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), significantly improving outcomes in both adults and children. By simultaneously binding to CD19 on B cells and CD3 on T cells, BLN triggers target cell-dependent T-cell activation, resulting in the cytolysis of CD19⁺ BCP-ALL cells. Despite the remarkable clinical advancements achieved with BLN, the immunological mechanisms underlying treatment response or failure remain poorly characterized. γδ T cells are attractive candidates for adoptive T-cell therapy due to potent cytotoxicity, capacity to present antigens, broad lysis of different tumor entities, and low alloreactivity. Because γδ T cells can also be redireFrontiers in immunology1739493https://www.ebi.ac.uk/biostudies/studies/S-EPMC12920462biostudies-literatureBlinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: insights from &lt;i&gt;in vitro&lt;/i&gt; assays.PMC12920462Bendig SPeters CKabelitz DNasr NLaqua AJanssen OTrautmann HBaldus CDChitadze GScheffold AWesch DKelm MLustig MOberg HHBruggemann MValerius TfalseBlinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: insights from &lt;i&gt;in vitro&lt;/i&gt; assays.<h4>Introduction</h4>Blinatumomab (BLN) is a bispecific T-cell engager that has revolutionized the treatment of B-cell precursor acute lymphoblastic leukemia (BCP-ALL), significantly improving outcomes in both adults and children. By simultaneously binding to CD19 on B cells and CD3 on T cells, BLN triggers target cell-dependent T-cell activation, resulting in the cytolysis of CD19⁺ BCP-ALL cells. Despite the remarkable clinical advancements achieved with BLN, the immunological mechanisms underlying treatment response or failure remain poorly characterized. γδ T cells are attractive candidates for adoptive T-cell therapy due to potent cytotoxicity, capacity to present antigens, broad lysis of different tumor entities, and low alloreactivity. Because γδ T cells can also be redire2026-01-01T00:00:00Z20262026-07-16T18:06:56.547Z2026-07-09T11:10:43.196ZS-EPMC129204624172747010.3389/fimmu.2026.1739493