{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17"],"submitter":["Xing J"],"pubmed_abstract":["Lysine-Histidine Transporter 1 (LHT1) is a key amino acid transporter involved in plant nutrition, metabolism, leaf development, and abiotic stress tolerance. However, the regulatory mechanisms of NtLHT1 (LHT1 in <i>Nicotiana tobacum</i>) across different plant organs remain poorly understood. In this study, we utilized <i>NtLHT1</i> knockout (LHT1-KO), overexpression (LHT1-OE), and wild-type (LHT1-WT) tobacco lines (cv. Honghuadajinyuan) to investigate the organ-specific effects of altered <i>NtLHT1</i> expression on metabolic and transcriptomic profiles. At the 10-leaf stage, leaves, stems, and roots were collected for transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis, with qRT-PCR performed to "],"journal":["Frontiers in plant science"],"pagination":["1663088"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12920487"],"repository":["biostudies-literature"],"pubmed_title":["Integrative transcriptomic and metabolomic analyses provide insights into the effects of overexpression and knockout of &lt;i&gt;NtLHT1&lt;/i&gt; in different tissues."],"pmcid":["PMC12920487"],"pubmed_authors":["Cheng C","Xu L","Gao Q","Xing J","Yang W","Jiang J","He W","Mi Q","Wang W","Xiang H","Zhang L","Zeng W"],"additional_accession":[]},"is_claimable":false,"name":"Integrative transcriptomic and metabolomic analyses provide insights into the effects of overexpression and knockout of &lt;i&gt;NtLHT1&lt;/i&gt; in different tissues.","description":"Lysine-Histidine Transporter 1 (LHT1) is a key amino acid transporter involved in plant nutrition, metabolism, leaf development, and abiotic stress tolerance. However, the regulatory mechanisms of NtLHT1 (LHT1 in <i>Nicotiana tobacum</i>) across different plant organs remain poorly understood. In this study, we utilized <i>NtLHT1</i> knockout (LHT1-KO), overexpression (LHT1-OE), and wild-type (LHT1-WT) tobacco lines (cv. Honghuadajinyuan) to investigate the organ-specific effects of altered <i>NtLHT1</i> expression on metabolic and transcriptomic profiles. At the 10-leaf stage, leaves, stems, and roots were collected for transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis, with qRT-PCR performed to ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026","modification":"2026-07-09T12:06:51.804Z","creation":"2026-07-09T11:10:27.725Z"},"accession":"S-EPMC12920487","cross_references":{"pubmed":["41727988"],"doi":["10.3389/fpls.2026.1663088"]}}