biostudies-literatureUnknown17Xing JLysine-Histidine Transporter 1 (LHT1) is a key amino acid transporter involved in plant nutrition, metabolism, leaf development, and abiotic stress tolerance. However, the regulatory mechanisms of NtLHT1 (LHT1 in <i>Nicotiana tobacum</i>) across different plant organs remain poorly understood. In this study, we utilized <i>NtLHT1</i> knockout (LHT1-KO), overexpression (LHT1-OE), and wild-type (LHT1-WT) tobacco lines (cv. Honghuadajinyuan) to investigate the organ-specific effects of altered <i>NtLHT1</i> expression on metabolic and transcriptomic profiles. At the 10-leaf stage, leaves, stems, and roots were collected for transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis, with qRT-PCR performed to Frontiers in plant science1663088https://www.ebi.ac.uk/biostudies/studies/S-EPMC12920487biostudies-literatureIntegrative transcriptomic and metabolomic analyses provide insights into the effects of overexpression and knockout of <i>NtLHT1</i> in different tissues.PMC12920487Cheng CXu LGao QXing JYang WJiang JHe WMi QWang WXiang HZhang LZeng WfalseIntegrative transcriptomic and metabolomic analyses provide insights into the effects of overexpression and knockout of <i>NtLHT1</i> in different tissues.Lysine-Histidine Transporter 1 (LHT1) is a key amino acid transporter involved in plant nutrition, metabolism, leaf development, and abiotic stress tolerance. However, the regulatory mechanisms of NtLHT1 (LHT1 in <i>Nicotiana tobacum</i>) across different plant organs remain poorly understood. In this study, we utilized <i>NtLHT1</i> knockout (LHT1-KO), overexpression (LHT1-OE), and wild-type (LHT1-WT) tobacco lines (cv. Honghuadajinyuan) to investigate the organ-specific effects of altered <i>NtLHT1</i> expression on metabolic and transcriptomic profiles. At the 10-leaf stage, leaves, stems, and roots were collected for transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based metabolomic analysis, with qRT-PCR performed to 2026-01-01T00:00:00Z20262026-07-09T12:06:51.804Z2026-07-09T11:10:27.725ZS-EPMC129204874172798810.3389/fpls.2026.1663088