biostudies-literatureUnknown9(1)Zhou HHighly-charged intrinsically disordered proteins (IDPs) underpin biomolecular condensate formation through liquid-liquid phase separation, yet the influence of charge sequences on the dynamics within the condensate phase remains poorly understood. Using extensive molecular dynamics simulations with explicit hydrodynamics and electrostatics, we study the dynamics in IDP condensates across different length and time scales, by systematically varying the charge sequences of the constituent IDPs. Contrary to the expectation that long-range interactions are heavily screened in dense semidilute polymer solutions, we find hydrodynamics and electrostatics significantly influence the dynamics in IDP condensates and their effects are strongly coupled to the charge sequence of the constituent IDPs. FoCommunications chemistry98https://www.ebi.ac.uk/biostudies/studies/S-EPMC12920641biostudies-literatureSequence and length-scale dependent dynamics in biocondensates of highly charged disordered proteins.PMC12920641Wu ZJiang LWang ZGChen SZhou HfalseSequence and length-scale dependent dynamics in biocondensates of highly charged disordered proteins.Highly-charged intrinsically disordered proteins (IDPs) underpin biomolecular condensate formation through liquid-liquid phase separation, yet the influence of charge sequences on the dynamics within the condensate phase remains poorly understood. Using extensive molecular dynamics simulations with explicit hydrodynamics and electrostatics, we study the dynamics in IDP condensates across different length and time scales, by systematically varying the charge sequences of the constituent IDPs. Contrary to the expectation that long-range interactions are heavily screened in dense semidilute polymer solutions, we find hydrodynamics and electrostatics significantly influence the dynamics in IDP condensates and their effects are strongly coupled to the charge sequence of the constituent IDPs. Fo2026-01-01T00:00:00Z2026 Jan2026-07-16T12:35:51.504Z2026-07-09T10:56:00.825ZS-EPMC129206414156603310.1038/s42004-026-01903-0