biostudies-literatureWatanabe TTokai University School of Medicine Research AidJapan Society for the Promotion of Science7112https://www.ebi.ac.uk/biostudies/studies/S-EPMC12920786biostudies-literatureUnknown16(1)Ultraviolet (UV) radiation is a major environmental factor that induces DNA lesions. Cells have evolved repair pathways, in which the transcription-coupled nucleotide excision repair (TC-NER) has a central role in removing the lesions. Here we demonstrate that DGCR8, known as a crucial component in microRNA biogenesis, coordinates the UV-induced formation of the TC-NER complex by interacting with TC-NER factors. These interactions could depend on the phosphorylation of Serine 153 of DGCR8, potentially serving as a functional switch from miRNA biogenesis to the TC-NER process. Interestingly, DGCR8 is also involved in recruiting chromatin remodelers, SPT16 and SMARCA5, for the TC-NER initiation, regulating UV-induced DNA/RNA hybrids (R-loops), and modulating DNA replication through the ATR-CScientific reportsDGCR8 regulates multiple processes of transcription coupled nucleotide excision repair.PMC129207862018, 2019, 2021 and 2023KAKENHI 15K21771, 23K21303KAKENHI 23K21303, 19K06493, 24K152922022 and 2023Tanaka YWatanabe TYamasaki HOhtsuka MTaniguchi TYoshinami DfalseDGCR8 regulates multiple processes of transcription coupled nucleotide excision repair.Ultraviolet (UV) radiation is a major environmental factor that induces DNA lesions. Cells have evolved repair pathways, in which the transcription-coupled nucleotide excision repair (TC-NER) has a central role in removing the lesions. Here we demonstrate that DGCR8, known as a crucial component in microRNA biogenesis, coordinates the UV-induced formation of the TC-NER complex by interacting with TC-NER factors. These interactions could depend on the phosphorylation of Serine 153 of DGCR8, potentially serving as a functional switch from miRNA biogenesis to the TC-NER process. Interestingly, DGCR8 is also involved in recruiting chromatin remodelers, SPT16 and SMARCA5, for the TC-NER initiation, regulating UV-induced DNA/RNA hybrids (R-loops), and modulating DNA replication through the ATR-C2026-01-01T00:00:00Z2026 Feb2026-07-16T12:59:26.66Z2026-07-09T10:57:00.35ZS-EPMC129207864163433610.1038/s41598-026-38338-5