{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song JS"],"funding":["Korean Association for the Study of the Liver, Gyeong-In Area","Asan Medical Center fund","Soonchunhyang University research fund"],"pagination":["16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12922343"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(1)"],"pubmed_abstract":["<h4>Objectives</h4>Pyogenic liver abscess (PLA) is a life-threatening infection with high mortality in Asia. Although Klebsiella pneumoniae is commonly implicated, emerging data suggest a more diverse microbial spectrum. This study investigated pathogen detection using conventional culture and next-generation sequencing (NGS) and characterized gut microbiome alterations in PLA patients compared to healthy controls.<h4>Method</h4>This was a prospective, multicenter cohort study conducted across eight tertiary hospitals. We enrolled 100 PLA patients who underwent percutaneous aspiration. Abscess aspirates underwent both conventional culture and 16 S rRNA-based NGS. Stool samples from PLA patients and 100 healthy controls were analyzed for gut microbiome composition using NGS.<h4>Results</h4>"],"journal":["Gut pathogens"],"pubmed_title":["Enhanced pathogen detection and gut microbiome alterations in pyogenic liver abscess: insights from next-generation sequencing."],"pmcid":["PMC12922343"],"funding_grant_id":["2020IF0005"],"pubmed_authors":["Han JE","Kim SS","Choi GH","Shin SK","Yoo JJ","Kweon S","Jang ES","Kang SH","Kim SG","Lee HL","Lee SW","Song JS","Yu JH","Kim MJ","Yim HJ","Kim YS","Jung YK"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced pathogen detection and gut microbiome alterations in pyogenic liver abscess: insights from next-generation sequencing.","description":"<h4>Objectives</h4>Pyogenic liver abscess (PLA) is a life-threatening infection with high mortality in Asia. Although Klebsiella pneumoniae is commonly implicated, emerging data suggest a more diverse microbial spectrum. This study investigated pathogen detection using conventional culture and next-generation sequencing (NGS) and characterized gut microbiome alterations in PLA patients compared to healthy controls.<h4>Method</h4>This was a prospective, multicenter cohort study conducted across eight tertiary hospitals. We enrolled 100 PLA patients who underwent percutaneous aspiration. Abscess aspirates underwent both conventional culture and 16 S rRNA-based NGS. Stool samples from PLA patients and 100 healthy controls were analyzed for gut microbiome composition using NGS.<h4>Results</h4>","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T13:02:16.763Z","creation":"2026-07-09T10:56:36.061Z"},"accession":"S-EPMC12922343","cross_references":{"pubmed":["41699716"],"doi":["10.1186/s13099-026-00799-4"]}}