biostudies-literatureSong JSKorean Association for the Study of the Liver, Gyeong-In AreaAsan Medical Center fundSoonchunhyang University research fund16https://www.ebi.ac.uk/biostudies/studies/S-EPMC12922343biostudies-literatureUnknown18(1)<h4>Objectives</h4>Pyogenic liver abscess (PLA) is a life-threatening infection with high mortality in Asia. Although Klebsiella pneumoniae is commonly implicated, emerging data suggest a more diverse microbial spectrum. This study investigated pathogen detection using conventional culture and next-generation sequencing (NGS) and characterized gut microbiome alterations in PLA patients compared to healthy controls.<h4>Method</h4>This was a prospective, multicenter cohort study conducted across eight tertiary hospitals. We enrolled 100 PLA patients who underwent percutaneous aspiration. Abscess aspirates underwent both conventional culture and 16 S rRNA-based NGS. Stool samples from PLA patients and 100 healthy controls were analyzed for gut microbiome composition using NGS.<h4>Results</h4>Gut pathogensEnhanced pathogen detection and gut microbiome alterations in pyogenic liver abscess: insights from next-generation sequencing.PMC129223432020IF0005Han JEKim SSChoi GHShin SKYoo JJKweon SJang ESKang SHKim SGLee HLLee SWSong JSYu JHKim MJYim HJKim YSJung YKfalseEnhanced pathogen detection and gut microbiome alterations in pyogenic liver abscess: insights from next-generation sequencing.<h4>Objectives</h4>Pyogenic liver abscess (PLA) is a life-threatening infection with high mortality in Asia. Although Klebsiella pneumoniae is commonly implicated, emerging data suggest a more diverse microbial spectrum. This study investigated pathogen detection using conventional culture and next-generation sequencing (NGS) and characterized gut microbiome alterations in PLA patients compared to healthy controls.<h4>Method</h4>This was a prospective, multicenter cohort study conducted across eight tertiary hospitals. We enrolled 100 PLA patients who underwent percutaneous aspiration. Abscess aspirates underwent both conventional culture and 16 S rRNA-based NGS. Stool samples from PLA patients and 100 healthy controls were analyzed for gut microbiome composition using NGS.<h4>Results</h4>2026-01-01T00:00:00Z2026 Feb2026-07-16T13:02:16.763Z2026-07-09T10:56:36.061ZS-EPMC129223434169971610.1186/s13099-026-00799-4