biostudies-literatureUnknown13(2)Falco-Roget A<h4>Background</h4>The diagnosis of <i>P. jirovecii</i> pneumonia (PJP) in immunosuppressed, HIV-negative patients is often limited by the inability to perform a bronchoalveolar lavage (BAL) due to respiratory insufficiency. In this study, we evaluated the diagnostic accuracy of combining serum beta-D-glucan (BDG) testing with <i>P. jirovecii</i> polymerase chain reaction (PCR) in oral wash (OW) samples as noninvasive tools for PJP diagnosis.<h4>Methods</h4>This was a retrospective, single-center study including all immunosuppressed non-HIV adult patients with suspected PJP in whom a Fujifilm Wako β-glucan test BDG and BAL PJ in-house PCR determination were performed during a 5-year period (2019-2024). The diagnostic performance (negative predictive value [NPV], positive predictive value [Open forum infectious diseasesofag033https://www.ebi.ac.uk/biostudies/studies/S-EPMC12923328biostudies-literatureCombined Serum (1,3)-β-D-Glucan and Oral Wash PCR as a Noninvasive Diagnostic Strategy for Early Detection of &lt;i&gt;Pneumocystis jirovecii&lt;/i&gt; Pneumonia: An Observational Retrospective Study.PMC12923328Falco-Roget ASerradell SRevilla EPerez-Gonzalez ALos-Arcos IDiaz-Lagares CRuiz-Camps IAlbasanz-Puig AGuillen ALen OMartin-Gomez MTfalseCombined Serum (1,3)-β-D-Glucan and Oral Wash PCR as a Noninvasive Diagnostic Strategy for Early Detection of &lt;i&gt;Pneumocystis jirovecii&lt;/i&gt; Pneumonia: An Observational Retrospective Study.<h4>Background</h4>The diagnosis of <i>P. jirovecii</i> pneumonia (PJP) in immunosuppressed, HIV-negative patients is often limited by the inability to perform a bronchoalveolar lavage (BAL) due to respiratory insufficiency. In this study, we evaluated the diagnostic accuracy of combining serum beta-D-glucan (BDG) testing with <i>P. jirovecii</i> polymerase chain reaction (PCR) in oral wash (OW) samples as noninvasive tools for PJP diagnosis.<h4>Methods</h4>This was a retrospective, single-center study including all immunosuppressed non-HIV adult patients with suspected PJP in whom a Fujifilm Wako β-glucan test BDG and BAL PJ in-house PCR determination were performed during a 5-year period (2019-2024). The diagnostic performance (negative predictive value [NPV], positive predictive value [2026-01-01T00:00:00Z2026 Feb2026-07-16T17:40:09.949Z2026-07-09T11:08:32.795ZS-EPMC129233284172570510.1093/ofid/ofag033