biostudies-literatureLu PStartup Fund for Scientific Research, Fujian Medical UniversityFujian Provincial Natural Science Foundation of Chinathe Joint Funds for the Innovation of Science and Technology, Fujian provinceFujian provincial health technology project348https://www.ebi.ac.uk/biostudies/studies/S-EPMC12923716biostudies-literatureUnknown17(1)<h4>Background</h4>Oxaliplatin (OXA) resistance poses a significant challenge to the efficacy of chemotherapy for advanced colorectal cancer (CRC). This study aimed to elucidate the role of OXA in regulating Cysteine-rich protein 61 (Cyr61) expression via the Hippo-Yes-associated protein (YAP) pathway in the context of chemotherapy-induced drug resistance.<h4>Methods</h4>We used Cell Counting Kit-8 to detect cell viability and proliferation. Expression of Cyr61 was determined by quantitative real-time PCR, western blotting (WB) and Enzyme-linked immunosorbent assay in CRC cell lines. The mechanism of OXA in regulating Cyr61 expression was studied by WB and co-immunoprecipitation.<h4>Results</h4>The results revealed that exposure to varying concentrations of OXA for 24 h induced increased mDiscover oncologyOxaliplatin upregulates Cyr61 expression by inhibiting the Hippo-YAP pathway which contributes to drug resistance of colorectal cancer cells.PMC129237162020Y90882021GGA0152024J015962023QH1057Zhang JHuang ZCao YZheng CZhu XChen XLu PfalseOxaliplatin upregulates Cyr61 expression by inhibiting the Hippo-YAP pathway which contributes to drug resistance of colorectal cancer cells.<h4>Background</h4>Oxaliplatin (OXA) resistance poses a significant challenge to the efficacy of chemotherapy for advanced colorectal cancer (CRC). This study aimed to elucidate the role of OXA in regulating Cysteine-rich protein 61 (Cyr61) expression via the Hippo-Yes-associated protein (YAP) pathway in the context of chemotherapy-induced drug resistance.<h4>Methods</h4>We used Cell Counting Kit-8 to detect cell viability and proliferation. Expression of Cyr61 was determined by quantitative real-time PCR, western blotting (WB) and Enzyme-linked immunosorbent assay in CRC cell lines. The mechanism of OXA in regulating Cyr61 expression was studied by WB and co-immunoprecipitation.<h4>Results</h4>The results revealed that exposure to varying concentrations of OXA for 24 h induced increased m2026-01-01T00:00:00Z2026 Jan2026-07-09T11:49:24.452Z2026-07-09T11:02:21.765ZS-EPMC129237164160621710.1007/s12672-026-04530-2