biostudies-literatureChu XNCI NIH HHSe70772https://www.ebi.ac.uk/biostudies/studies/S-EPMC12924702biostudies-literatureUnknown98(1)Kaposi sarcoma (KS) is an endothelial cancer caused by the Kaposi sarcoma-associated herpesvirus (KSHV), and it remains one of the most frequently diagnosed and fatal cancers in sub-Saharan Africa. Achieving timely and accurate diagnosis for KS is a critical challenge in sub-Saharan Africa, where access to histopathology is limited and clinical diagnosis is often erroneous. Previously, a point-of-care device was developed to quantify KSHV DNA and demonstrated promising performance, achieving 97% sensitivity and 92% specificity at a 26-min threshold in a training set of patients from several Ugandan clinics. Herein, 421 skin biopsies from a different, test set of patients identified later in calendar time at the same clinical sites were examined using the same methodology. Using the previouJournal of medical virologyLoop-Mediated Isothermal Amplification Enables Reliable Kaposi Sarcoma Diagnosis Across Time and Sites in East Africa.PMC12924702U01 CA269199Semeere ABoza JMMaurer TMuwando HChu XWenger MCesarman EAyanga RManning JCMartin JLukande RErickson DMcgaskey MfalseLoop-Mediated Isothermal Amplification Enables Reliable Kaposi Sarcoma Diagnosis Across Time and Sites in East Africa.Kaposi sarcoma (KS) is an endothelial cancer caused by the Kaposi sarcoma-associated herpesvirus (KSHV), and it remains one of the most frequently diagnosed and fatal cancers in sub-Saharan Africa. Achieving timely and accurate diagnosis for KS is a critical challenge in sub-Saharan Africa, where access to histopathology is limited and clinical diagnosis is often erroneous. Previously, a point-of-care device was developed to quantify KSHV DNA and demonstrated promising performance, achieving 97% sensitivity and 92% specificity at a 26-min threshold in a training set of patients from several Ugandan clinics. Herein, 421 skin biopsies from a different, test set of patients identified later in calendar time at the same clinical sites were examined using the same methodology. Using the previou2026-01-01T00:00:00Z2026 Jan2026-07-16T14:44:04.649Z2026-07-09T11:00:29.272ZS-EPMC129247024143979110.1002/jmv.70772