{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kumar A"],"funding":["National Institutes of Health","National Science Foundation"],"pagination":["104370"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12925548"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations."],"journal":["STAR protocols"],"pubmed_title":["Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes."],"pmcid":["PMC12925548"],"funding_grant_id":["2046984","R01AG058741"],"pubmed_authors":["Kumar A","Bai H"],"additional_accession":[]},"is_claimable":false,"name":"Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.","description":"Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-12T03:16:13.52Z","creation":"2026-07-12T03:08:00.674Z"},"accession":"S-EPMC12925548","cross_references":{"pubmed":["41701574"],"doi":["10.1016/j.xpro.2026.104370"]}}