biostudies-literatureKumar ANational Institutes of HealthNational Science Foundation104370https://www.ebi.ac.uk/biostudies/studies/S-EPMC12925548biostudies-literatureUnknown7(1)Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.STAR protocolsProtocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.PMC129255482046984R01AG058741Kumar ABai HfalseProtocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.2026-01-01T00:00:00Z2026 Feb2026-07-12T03:16:13.52Z2026-07-12T03:08:00.674ZS-EPMC129255484170157410.1016/j.xpro.2026.104370